{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:09:25Z","timestamp":1775768965559,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T00:00:00Z","timestamp":1488931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Minimally invasive diagnostic procedures such as needle\u2010core biopsy and fine\u2010needle aspiration provide adequate material for molecular analyses. Advances in precision oncology are trending toward the interrogation of limited amounts of genomic material to guide clinical and therapeutic decisions. The aim of this study was to investigate the minimum cellularity needed on cytologic smears for the identification of clinically relevant variants with next\u2010generation sequencing (NGS).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Thirty cases of cytologically diagnosed, resection\u2010proven primary lung adenocarcinoma were identified. Nineteen of the 30 cases were known to harbor actionable variants. One Diff\u2010Quik (DQ)\u2013stained slide and 1 Papanicolaou (Pap)\u2010stained slide were selected from each case. Cases were categorized as containing fewer than 100 tumor cells, 100 to 500 tumor cells, or more than 500 tumor cells. NGS was performed on the Ion Torrent platform.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>NGS was successfully performed on all cell blocks and on 90% of the smears. Paired DQ and Pap smears showed similar cellularity, and cases that differed in cellularity were within 1 category of each other. The cases with more than 100 tumor cells had a 93% success rate; this was significantly different from the situation for cases with fewer than 100 tumor cells, which were successfully sequenced only 67% of the time. Overall, NGS was able to provide clinically relevant information for 83% of DQ smears and for 90% of Pap smears tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The data show a significantly higher likelihood of successful NGS with cytologic smears with more than 100 tumor cells. There was a trend for a higher NGS success rate with Pap smears versus DQ smears. <jats:bold><jats:italic>Cancer Cytopathol<\/jats:italic> 2017;125:398\u2013406<\/jats:bold>. \u00a9 <jats:italic>2017 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21844","type":"journal-article","created":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T09:52:41Z","timestamp":1488966761000},"page":"398-406","source":"Crossref","is-referenced-by-count":40,"title":["Accuracy of next\u2010generation sequencing for the identification of clinically relevant variants in cytology smears in lung adenocarcinoma"],"prefix":"10.1002","volume":"125","author":[{"given":"Jordan E.","family":"Baum","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Weill Cornell Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of 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