{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:57:13Z","timestamp":1790740633046,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T00:00:00Z","timestamp":1518739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Rearrangements of the <jats:italic>ROS1<\/jats:italic> oncogene are found in 1% to 2% of non\u2010small cell lung cancers (NSCLC) and are regarded as mutually exclusive oncogenic driver mutations. Since the approval of targeted therapy for ROS1\u2010positive NSCLC, ROS1 testing has become a part of the diagnostic routine. Fluorescence in situ hybridization (FISH), optionally selected for by immunohistochemistry on histological material, is a common practice for the detection of <jats:italic>ROS1<\/jats:italic> rearrangements. However, NSCLC often is diagnosed by cytology alone, requiring predictive marker testing on cytological specimens. In the current study, the authors explored the accuracy of ROS1 immunocytochemistry (ICC) on non\u2010cell block cytological specimens for the detection of <jats:italic>ROS1<\/jats:italic> rearrangements.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>ICC using the D4D6 antibody on an automated immunostainer was performed prospectively in the routine diagnostic setting on cytological specimens from 295 patients with NSCLC, including adenocarcinoma (241 patients), NSCLC not otherwise specified (50 patients), and other malignancies (4 patients). Any immunostaining was considered positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>ICC was positive in all 13 <jats:italic>ROS1<\/jats:italic>\u2010rearranged NSCLC cases confirmed by FISH (12 cases) or next\u2010generation sequencing (1 case). Confirmation of 282 ICC\u2010negative cases was available for 208 patients. The sensitivity, specificity, and positive and negative predictive values for ROS1 ICC compared with the final ROS1 status all were 100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>ROS1 ICC is an accurate method for the detection of <jats:italic>ROS1<\/jats:italic> rearrangements in NSCLC. Given the high costs and technical challenges of FISH and the rarity of <jats:italic>ROS1<\/jats:italic> rearrangements, ICC is rapid and therefore well suited as a screening method. Cases with equivocal or positive findings on ICC can be confirmed by FISH or molecular tests. <jats:bold><jats:italic>Cancer Cytopathol<\/jats:italic> 2018;126:421\u20109.<\/jats:bold> \u00a9 <jats:italic>2018 American Cancer Society<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.21983","type":"journal-article","created":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T09:56:50Z","timestamp":1518775010000},"page":"421-429","source":"Crossref","is-referenced-by-count":37,"title":["Detection of ROS1\u2010positive non\u2010small cell lung cancer on cytological specimens using immunocytochemistry"],"prefix":"10.1002","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-6771","authenticated-orcid":false,"given":"Tatjana","family":"Vlajnic","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital Basel Basel 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