{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:38:52Z","timestamp":1790307532848,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T00:00:00Z","timestamp":1583971200000},"content-version":"am","delay-in-days":377,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["OC100517"],"award-info":[{"award-number":["OC100517"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P50 CA228991"],"award-info":[{"award-number":["P50 CA228991"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The paradigm shift localizing the origin of ovarian high\u2010grade serous carcinoma (HGSC) to the fallopian tube underscores the rationale for meticulous microscopic examination of salpingectomy specimens. The precursor, termed \u201cserous tubal intraepithelial carcinoma,\u201d is often a focal lesion, which poses difficulties for histologic diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The authors describe a method to examine exfoliated epithelial cells from fallopian tube fimbria by gentle brushing, thereby enabling thorough sampling of the mucosal surface. Fimbrial brushings were collected from 20 fresh salpingectomy specimens from 15 patients, including 5 who had pathologically confirmed ovarian HGSC. Samples taken only from tubes that were grossly negative for tumor were processed for Papanicolaou staining, p53 immunocytochemistry, and tumor protein 53 (<jats:italic>TP53<\/jats:italic>) mutation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cells with malignant cytomorphologic features were identified only in tubal brushings from patients with ovarian HGSC. In all cases, atypical\/malignant cells on cytology corresponded to lesions with similar morphology and immunostaining pattern in permanent sections, demonstrating the sensitivity of the technique while providing reassurance that specimen integrity was not disrupted by the procedure. Targeted next\u2010generation sequencing confirmed the presence of <jats:italic>TP53<\/jats:italic> mutations in fimbrial brushings from HGSC, but not in benign samples, and demonstrated concordance with the immunostaining pattern. Identical mutations were observed in matched lesions microdissected from formalin\u2010fixed tissue sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The described technique enables cytologic evaluation of the fallopian tube fimbria for a diagnosis of serous tubal intraepithelial carcinoma, serving as a complement to histology while offering distinct advantages with respect to the procurement of cellular material for ancillary testing and research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22110","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T16:36:02Z","timestamp":1552408562000},"page":"192-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cytomorphologic and molecular analyses of fallopian tube fimbrial brushings for diagnosis of serous tubal intraepithelial carcinoma"],"prefix":"10.1002","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7220-3996","authenticated-orcid":false,"given":"M. Herman","family":"Chui","sequence":"first","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions Baltimore Maryland"},{"name":"Sidney Kimmel Cancer Center Johns Hopkins Medical Institutions Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell","family":"Vang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions Baltimore Maryland"},{"name":"Department of Gynecology and Obstetrics Johns Hopkins University School of Medicine Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian\u2010Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions Baltimore Maryland"},{"name":"Sidney Kimmel Cancer Center Johns Hopkins Medical Institutions Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ie\u2010Ming","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions Baltimore Maryland"},{"name":"Department of Gynecology and Obstetrics Johns Hopkins University School of Medicine Baltimore Maryland"},{"name":"Sidney Kimmel Cancer Center Johns Hopkins Medical Institutions Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-5655","authenticated-orcid":false,"given":"Christopher J.","family":"VandenBussche","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions Baltimore 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cytology of the fallopian tube as a promising tool for ovarian cancer early detection","volume":"125","author":"Chen H","year":"2017","journal-title":"J Vis Exp"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/pgp.0b013e31814b191f"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356988"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djx225"}],"container-title":["Cancer 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