{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:27:58Z","timestamp":1789612078998,"version":"build-2803163510"},"reference-count":80,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T00:00:00Z","timestamp":1556064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>The Afirma Genomic Sequencing Classifier (GSC) is a rule\u2010out test for malignancy\/noninvasive follicular thyroid neoplasms with papillary\u2010like nuclear features among patients with Bethesda category III\/IV nodules, whereas the complimentary Xpression Atlas provides genomic insights from a curated panel of 511 genes among GSC suspicious and Bethesda category V\/VI nodules. Together, they facilitate personalized treatment decisions based on genomic insights derived from the transcriptome of the biopsied target and extend the diagnostic and therapeutic reach of cytopathologists and fine\u2010needle aspiration biopsy sample collection.<\/jats:p>","DOI":"10.1002\/cncy.22132","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T10:39:11Z","timestamp":1556102351000},"page":"362-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Extending expressed RNA genomics from surgical decision making for cytologically indeterminate thyroid nodules to targeting therapies for metastatic thyroid cancer"],"prefix":"10.1002","volume":"127","author":[{"given":"Syed Z.","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Pathology The Johns Hopkins Hospital Baltimore Maryland"},{"name":"Department of Radiology The Johns Hopkins Hospital Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Siperstein","sequence":"additional","affiliation":[{"name":"Department of Endocrine Surgery Cleveland Clinic Cleveland Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Sadow","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital, Harvard Medical School Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan C.","family":"Golding","sequence":"additional","affiliation":[{"name":"Department of Medicine Florida International University, Memorial Healthcare System Hollywood Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia C.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Department of Clinical Affairs Veracyte Inc South San Francisco California"},{"name":"Department of Medical Affairs Veracyte Inc South San Francisco California"},{"name":"Department of Research and Development Veracyte Inc South San Francisco California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4183-7387","authenticated-orcid":false,"given":"Richard T.","family":"Kloos","sequence":"additional","affiliation":[{"name":"Department of Medical Affairs Veracyte Inc South San Francisco California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"Ladenson","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, and Metabolism Department of Medicine Johns Hopkins University School of Medicine Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,24]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2016.0280"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-01674"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0154-SA"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000339959"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPRA9Y2XQVFOFV"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21468"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21624"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP4P2GCCDNHFMY"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000441221"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-011-9159-6"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2015.2708"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/000313481808400740"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0655-3016"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-18-0078"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2018.0085"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2018.04.094"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2010.0243"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203208"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199809000-00005"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2015.2503"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.26119"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000001688"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-013589"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13895"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10843"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2017.3378"},{"key":"e_1_2_4_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2018.0067"},{"key":"e_1_2_4_29_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2016.63004"},{"key":"e_1_2_4_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2017.04.035"},{"key":"e_1_2_4_31_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2017.4593"},{"key":"e_1_2_4_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2018.04.013"},{"key":"e_1_2_4_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2018.03.057"},{"key":"e_1_2_4_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20092"},{"key":"e_1_2_4_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-1087"},{"key":"e_1_2_4_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-2658"},{"key":"e_1_2_4_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25607"},{"key":"e_1_2_4_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2012.0180"},{"key":"e_1_2_4_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2482"},{"key":"e_1_2_4_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21455"},{"key":"e_1_2_4_41_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3584"},{"key":"e_1_2_4_42_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-015-4486-3"},{"key":"e_1_2_4_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2015.0505"},{"key":"e_1_2_4_44_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP151006.OR"},{"key":"e_1_2_4_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23378"},{"key":"e_1_2_4_46_1","doi-asserted-by":"publisher","DOI":"10.2214\/AJR.14.13984"},{"key":"e_1_2_4_47_1","doi-asserted-by":"publisher","DOI":"10.1185\/03007995.2016.1166103"},{"key":"e_1_2_4_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-016-0263-4"},{"key":"e_1_2_4_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599816652378"},{"key":"e_1_2_4_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23559"},{"key":"e_1_2_4_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21749"},{"key":"e_1_2_4_52_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2016.3526"},{"key":"e_1_2_4_53_1","doi-asserted-by":"publisher","DOI":"10.1159\/000446797"},{"key":"e_1_2_4_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40463-017-0201-7"},{"key":"e_1_2_4_55_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0328-OA"},{"key":"e_1_2_4_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21879"},{"key":"e_1_2_4_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21827"},{"key":"e_1_2_4_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-017-1509-9"},{"key":"e_1_2_4_59_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP161231.OR"},{"key":"e_1_2_4_60_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP-2018-0395"},{"key":"e_1_2_4_61_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-159-5-201309030-00006"},{"key":"e_1_2_4_62_1","article-title":"Outcomes of Bethesda categories III and IV thyroid nodules over 5 years and performance of the Afirma gene expression classifier: a single\u2010institution study [published online May 23, 2018]","author":"Deaver KE","journal-title":"Clin Endocrinol (Oxf)"},{"key":"e_1_2_4_63_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2016.0656"},{"key":"e_1_2_4_64_1","article-title":"Afirma Genomic Sequencing Classifier experience among the first 1614 consecutive samples from cytologically indeterminate thyroid nodules [abstract]","volume":"39","author":"Shanik MH","year":"2018","journal-title":"Endocr Rev"},{"key":"e_1_2_4_65_1","first-page":"A","article-title":"Practical clinical use of Afirma thyroid FNA analysis for indeterminate thyroid nodules within the context of cytology and TIRADS score (poster 445)","volume":"28","author":"Bobanga I","year":"2018","journal-title":"Thyroid"},{"key":"e_1_2_4_66_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2018.1153"},{"key":"e_1_2_4_67_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12918-019-0693-z"},{"key":"e_1_2_4_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-6-10"},{"key":"e_1_2_4_69_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1530-891X(20)47521-4","article-title":"High Afirma GSC benign call\u2010rate and estimated negative predictive value [abstract]","volume":"24","author":"Ahmed S","year":"2018","journal-title":"Endocr Pract"},{"key":"e_1_2_4_70_1","first-page":"A","article-title":"Experience with the Genomic Sequencing Classifier in >100 cytologically indeterminate thyroid nodules (short call poster 33)","volume":"28","author":"Endo M","year":"2018","journal-title":"Thyroid"},{"key":"e_1_2_4_71_1","first-page":"A","article-title":"Performance of Afirma Genomic Sequencing Classifier: the initial Cleveland Clinic experience (poster 450)","volume":"28","author":"San Martin VT","year":"2018","journal-title":"Thyroid"},{"key":"e_1_2_4_72_1","first-page":"A","article-title":"Genomic sequencing classifier versus ThyroSeq v3 in the management of indeterminate thyroid nodules: a randomized clinical trial (short call poster 44)","volume":"28","author":"Livhits MJ","year":"2018","journal-title":"Thyroid"},{"key":"e_1_2_4_73_1","article-title":"An independent comparison of the Afirma genomic sequencing classifier (GSC) and gene expression classifier (GEC) for cytologically indeterminate thyroid nodules [published online February 26, 2019]","author":"Angell TE","journal-title":"Thyroid"},{"key":"e_1_2_4_74_1","article-title":"Detection of increasing numbers of point mutations and fusions in thyroid nodules using next\u2010generation sequencing dramatically reduces marker panel specificity [abstract]","volume":"36","author":"Kennedy GC","year":"2015","journal-title":"Endocr Rev"},{"key":"e_1_2_4_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-015-0849-9"},{"key":"e_1_2_4_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.050"},{"key":"e_1_2_4_77_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-0373"},{"key":"e_1_2_4_78_1","article-title":"Performance of a multigene genomic classifier in thyroid nodules with indeterminate cytology: a prospective blinded multicenter study [published online November 8, 2018]","author":"Steward DL","journal-title":"JAMA Oncol"},{"key":"e_1_2_4_79_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006239"},{"key":"e_1_2_4_80_1","first-page":"A","article-title":"Clinical activity of LOXO\u2010292, a highly selective RET inhibitor, in patients with RET\u2010altered thyroid cancers (short call oral 6)","volume":"28","author":"Wirth LJ","year":"2018","journal-title":"Thyroid"},{"key":"e_1_2_4_81_1","first-page":"A","article-title":"Clinical activity of selective RET inhibitor, BLU\u2010667, in advanced RET\u2010altered thyroid cancers: updated results from the phase 1 ARROW study (short call oral 5)","volume":"28","author":"Hu M","year":"2018","journal-title":"Thyroid"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22132","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncy.22132","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22132","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T12:55:57Z","timestamp":1760273757000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.22132"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,24]]},"references-count":80,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/cncy.22132"],"URL":"https:\/\/doi.org\/10.1002\/cncy.22132","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,24]]},"assertion":[{"value":"2019-02-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}