{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:48:02Z","timestamp":1791571682478,"version":"4.3.4"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T00:00:00Z","timestamp":1565308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA008748"],"award-info":[{"award-number":["P30CA008748"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The Ki\u201067 proliferation marker has multiple diagnostic and prognostic applications. Although several clones to the Ki\u201067 antigen are commercially available, the MIB1 clone is widely recommended in the surgical pathology literature for neuroendocrine tumors. In our cytopathology practice, we have encountered unexpectedly low MIB1 immunoreactivity in CytoLyt\u2010fixed cell blocks (CBs). The current study evaluated the impact of fixatives, CB processing, and immunocytochemical (ICC) procedures on Ki\u201067 immunoreactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Test CBs were prepared from freshly resected tumors, and multiple variables in the MIB1 ICC procedure were tested, including CytoLyt versus formalin collection media, MIB1 versus other Ki\u201067 clones including 30\u20109, and other variables. MIB1 versus Ki\u201067 30\u20109 clones were tested in parallel on CytoLyt\u2010fixed CBs from clinical samples of small cell lung carcinoma (SCLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the test CBs (n\u00a0=\u00a010), the mean MIB1 labeling index was 10% in CytoLyt versus 47% in formalin (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.0116), with a mean loss of reactivity in matched CBs of 37% (up to 70%). None of the procedure modifications tested in 223 individual ICC reactions recovered MIB1 reactivity in CytoLyt except for switching to the Ki\u201067 30\u20109 antibody. In CytoLyt\u2010fixed SCLC samples (n\u00a0=\u00a014), the Ki\u201067 30\u20109 antibody demonstrated expected ranges of reactivity (mean, 83%; range, 60%\u2010100%), whereas MIB1 demonstrated markedly inhibited labeling (mean, 60%; range, 10%\u201095%) (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.0058).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CytoLyt fixation substantially inhibits MIB1 immunoreactivity, whereas the Ki\u201067 30\u20109 clone is not susceptible to inhibition. Markedly discrepant MIB1 reactivity may present a pitfall in the diagnosis of SCLC and may lead to the incorrect prognostic stratification of other tumor types. For laboratories using CytoLyt, we recommend using the Ki\u201067 30\u20109 antibody rather than the MIB1 antibody.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22170","type":"journal-article","created":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T12:04:56Z","timestamp":1565352296000},"page":"643-649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["CytoLyt fixation significantly inhibits MIB1 immunoreactivity whereas alternative Ki\u201067 clone 30\u20109 is not susceptible to the inhibition: Critical diagnostic implications"],"prefix":"10.1002","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2035-8440","authenticated-orcid":false,"given":"Darren J.","family":"Buonocore","sequence":"first","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New 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