{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:47:59Z","timestamp":1791571679034,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T00:00:00Z","timestamp":1570665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA008748"],"award-info":[{"award-number":["P30CA008748"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The management of high\u2010risk human papillomavirus (HR\u2010HPV)\u2013related oropharyngeal head and neck squamous cell carcinomas (HNSCCs) are distinct from HNSCC linked to smoking and alcohol use. HR\u2010HPV\u2013positive HNSCC frequently presents as a cervical lymph node metastasis. Because fine\u2010needle aspiration (FNA) is often the initial diagnostic procedure, evaluating HR\u2010HPV status in cytology specimens is important. The overexpression of p16 is a surrogate for HR\u2010HPV; however, the evaluation of p16 in FNAs remains controversial.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From September 2015 to December 2016, cytopathologists performed 25 FNAs of neck lymph nodes that were suspicious for HR\u2010HPV\u2013positive HNSCC. Initial passes produced smears for on\u2010site evaluation and CytoLyt material. Additional passes were formalin\u2010fixed. A CytoLyt cell block (CCB) and a formalin\u2010fixed cell block (FFCB) were prepared, and p16 immunocytochemistry was performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In 24 of 25 cases, the FFCB had diffuse (\u226570% of cells), strong nuclear\/cytoplasmic p16 staining. In all 24 of these cases, HR\u2010HPV was detected by in situ hybridization. The corresponding CCB had weak\u2010to\u2010moderate p16 staining in &lt;70% of cells (range, 5%\u201060% of cells) in 17 cases, 4 had weak\u2010to\u2010moderate diffuse staining, and 4 were acellular. The percentage of p16\u2010positive cells was significantly higher with FFCB than with CCB (formalin: 94%\u00a0\u00b1\u00a02%; CytoLyt, 38%\u00a0\u00b1\u00a07%; 2\u2010tailed, paired Student\n                      <jats:italic>t<\/jats:italic>\n                      test;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001; Fisher exact test,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The fixative used had a drastic impact on p16 staining, which explained the staining variability reported in the literature. FFCBs show a diffuse staining pattern, which correlates with HR\u2010HPV status, whereas CCBs show a weaker and inconsistent staining pattern, which is more difficult to interpret.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22191","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T14:53:53Z","timestamp":1570719233000},"page":"750-756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Cytologic evaluation of p16 staining in head and neck squamous cell carcinoma in CytoLyt versus formalin\u2010fixed material"],"prefix":"10.1002","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2035-8440","authenticated-orcid":false,"given":"Darren J.","family":"Buonocore","sequence":"first","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Fowle","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"},{"name":"Department of Pathology OU Medical Center Oklahoma City Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2089-6118","authenticated-orcid":false,"given":"Oscar","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4638-9835","authenticated-orcid":false,"given":"Bin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-3046","authenticated-orcid":false,"given":"Nora","family":"Katabi","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0708-5919","authenticated-orcid":false,"given":"Jean\u2010Marc","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23014"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.04.002"},{"key":"e_1_2_8_4_1","unstructured":"PfisterDG SpencerS AdelsteinD et al.NCCN Clinical Practice Guidelines in Oncology. 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