{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:36:19Z","timestamp":1788176179576,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003852","name":"Regione Campania","doi-asserted-by":"publisher","award":["LR n.24 29\/12\/2005."],"award-info":[{"award-number":["LR n.24 29\/12\/2005."]}],"id":[{"id":"10.13039\/501100003852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Thyroid fine\u2010needle aspiration (FNA) is a reliable and cost\u2010effective diagnostic tool for establishing the nature of thyroid nodules, although up to 30% of FNAs are still classified as \u201cindeterminate.\u201d Molecular testing of FNAs could improve preoperative diagnosis, thereby reducing unnecessary surgery. In this multicenter prospective study\u00a0the authors investigated,\u00a0using a 7\u2010gene assay, the distribution and diagnostic impact of <jats:italic>BRAF<\/jats:italic>, <jats:italic>RAS<\/jats:italic>, <jats:italic>RET\/PTC<\/jats:italic>, and <jats:italic>PAX8\/PPARg<\/jats:italic>, the\u00a0most frequent genomic alterations occurring during thyroid oncogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, of 1172 routine FNAs from 7 centers in southern Italy were classified according to the Bethesda System for Reporting Thyroid Cytopathology. Each specimen was tested, and molecular data were compared with available histology or cytologic follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In particular, for atypia of undetermined significance\/follicular lesion of undetermined significance cases, the 7\u2010gene test confirmed the high positive predictive value of <jats:italic>BRAF<\/jats:italic>V600E and <jats:italic>BRAF<\/jats:italic>\u2010like mutations (80%) and the moderate positive predictive value of <jats:italic>RAS<\/jats:italic>\u2010like alterations (32.4%), suggesting different surgical management, depending on the type of mutation. The rate of mutation\u2010positive FNAs was strictly related to the risk of malignancy of each diagnostic class, supporting the identification of prognostically relevant diagnostic categories.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The 7\u2010gene panel test improves the preoperative risk stratification of indeterminate thyroid FNAs, especially when considering the biologic significance of the different types of mutations. Moreover, the rate of mutation\u2010positive FNAs is related to the risk of malignancy of each diagnostic class.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22217","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T11:10:22Z","timestamp":1575976222000},"page":"107-118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Evaluation of <i>BRAF<\/i>, <i>RAS<\/i>, <i>RET\/PTC<\/i>, and <i>PAX8\/PPARg<\/i> alterations in different Bethesda diagnostic categories: A multicentric prospective study on the validity of the 7\u2010gene panel test in 1172 thyroid FNAs deriving from different hospitals in South Italy"],"prefix":"10.1002","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7479-6457","authenticated-orcid":false,"given":"Claudio","family":"Bellevicine","sequence":"first","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Migliatico","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Sgariglia","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariantonia","family":"Nacchio","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Vigliar","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-0620","authenticated-orcid":false,"given":"Pasquale","family":"Pisapia","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"Iaccarino","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Bruzzese","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Fonderico","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine and Surgery University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Salvatore","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"Biondi","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine and Surgery University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Masone","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine and Surgery University of Naples Federico II Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Novizio","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Aziena Ospedaliera di Rilievo Nazionale A. Cardarelli Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Scavuzzo","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Aziena Ospedaliera di Rilievo Nazionale A. Cardarelli Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Serino","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Aziena Ospedaliera di Rilievo Nazionale A. Cardarelli Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"De Palma","sequence":"additional","affiliation":[{"name":"Department of Surgery Aziena Ospedaliera di Rilievo Nazionale A. Cardarelli Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Chiofalo","sequence":"additional","affiliation":[{"name":"Thyroid and Parathyroid Surgery Unit IRCCS G. 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