{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:57:44Z","timestamp":1767851864851,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T00:00:00Z","timestamp":1577404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatoblastoma (HB) is the most common malignant pediatric liver tumor, and cytology material is often the only tissue available for evaluation before definitive therapy. Subcategorization of HB based on cytomorphological features thus carries an important role in its prognostication. Spalt\u2010like transcription factor 4 (SALL4), a marker of embryonic stem cells that is also found in the fetal liver, is reactivated in certain liver tumors. Limited studies have evaluated its role in HB. This study was aimed at evaluating the cytomorphological features of HB and assessing the utility of SALL4 immunocytochemistry (ICC) in its subtyping and prognostication.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pretherapy fine\u2010needle aspiration smears from patients diagnosed with HB over a period of 9\u00a0years were retrieved. Aspirates were subclassified on the basis of the cytomorphology and were correlated with the histology wherever it was available. ICC for SALL4 was performed in 33 cases, and nuclear staining was considered positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 53 HB cases were included with 30 available postchemotherapy resection specimens. All the patients were diagnosed as\u00a0epithelial HB on cytology, and the cases were subclassified as pure fetal (9 of 53), pure embryonal (2 of 53), or combined epithelial HB (42 of 53). There was good concordance between cytology and histology for subtyping. SALL4 immunostaining displayed strong and diffuse nuclear positivity in the embryonal component while\u00a0focal and weak to negative staining in fetal cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Fine\u2010needle aspiration cytology serves as a rapid and effective tool for a correct diagnosis of HB before the implementation of chemotherapy, and SALL4 may serve as a useful diagnostic and prognostic marker.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22231","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T09:14:40Z","timestamp":1577438080000},"page":"190-200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cytomorphology of hepatoblastoma with histological correlation and role of SALL4 immunocytochemistry in its diagnosis, subtyping, and prognostication"],"prefix":"10.1002","volume":"128","author":[{"given":"Tripti","family":"Nakra","sequence":"first","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maitrayee","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Pathology Maharishi Markandeshwar Ambala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7597-4895","authenticated-orcid":false,"given":"Rajni","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Agarwala","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Jassim","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaurav","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasenjit","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5315-9814","authenticated-orcid":false,"given":"Deepali","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep R.","family":"Mathur","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkateswaran K.","family":"Iyer","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23642"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-008-1106-7"},{"key":"e_1_2_8_5_1","first-page":"497","volume-title":"Diagnostic Histopathology of Tumors","author":"Ferrell L","year":"2013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(00)00074-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12187"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(94)90453-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-014-1646-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.529"},{"key":"e_1_2_8_11_1","first-page":"1459","article-title":"Up\u2010regulation of SALL4 associated with poor prognosis in gastric cancer","volume":"61","author":"Liu J","year":"2014","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326166"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000333897"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21772"},{"key":"e_1_2_8_15_1","first-page":"331","article-title":"Fine needle aspiration cytology of hepatoblastoma: a study of 20 cases","volume":"48","author":"Parikh B","year":"2005","journal-title":"Indian J Pathol Microbiol"},{"key":"e_1_2_8_16_1","volume-title":"World Health Organization Classification of Tumours of the Digestive System","author":"Bosman FT","year":"2009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2010.00739.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2009.00732.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12627"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-9261.129602"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9371.117643"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-3151-9.00017-7"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.009"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.11.018"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.10.017"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2005.12.015"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12730"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-011-9364-0"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000116"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.22231","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22231","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncy.22231","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T09:32:02Z","timestamp":1760261522000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.22231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,27]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1002\/cncy.22231"],"URL":"https:\/\/doi.org\/10.1002\/cncy.22231","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,27]]},"assertion":[{"value":"2019-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}