{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T18:13:35Z","timestamp":1760292815284,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T00:00:00Z","timestamp":1591056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"crossref","award":["GR-2011-02349732"],"award-info":[{"award-number":["GR-2011-02349732"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>No screening is available for human papillomavirus (HPV)\u2010associated oropharyngeal cancers. The authors investigated whether cytology may be used as a screening tool and whether oral HPV infection is associated with cytologic abnormalities detected in oropharyngeal brushings and in oral rinse\u2010and\u2010gargle specimens from asymptomatic individuals at increased risk for oral HPV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Specimens were collected from men who have sex with men at 6\u2010month intervals. Oropharyngeal samples and oral rinse\u2010and\u2010gargle specimens were collected using a cytobrush and mouthwash, respectively. Exfoliated cells were dispersed in PreservCyt. Liquid\u2010based slides were stained with Papanicolaou. An HPV genotyping test using a linear array was used for HPV detection. Associations with abnormal cytology were investigated using logistic regression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 631 brushings and 802 rinses collected from 310 individuals were evaluated; of these\u00a0specimens, 2 brushings (0.3%) and 10 rinses (1.2%) were inadequate for morphologic evaluation. Of the adequate samples, 35 of 629 brushings (5.5%) and 19 of 792 rinses (2.4%) were abnormal. No associations of high\u2010risk HPVs or HPV\u201016 infection with cytologic abnormalities were observed for oropharyngeal brushings (high\u2010risk HPVs: odds ratio [OR], 1.19; 95% CI, 0.41\u20103.50; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.75; HPV\u201016: OR, 0.76; 95% CI, 0.10\u20105.84; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.79) or for oral rinses (high\u2010risk HPVs: OR, 1.13; 95% CI, 0.26\u20104.98; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.87; HPV\u201016: OR, 0.62; 95% CI, 0.04\u201010.60; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.74). Concurrent moderate\/heavy drinking and smoking significantly increased the risk of cytologic abnormalities in the brushings (hazard ratio, 4.84; 95% CI, 1.15\u201020.43; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.03).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oral HPV infection by high\u2010risk HPVs and HPV\u201016 does not confer an increased risk of cytologic abnormalities in oropharyngeal brushings and oral rinses. Abnormal cytology seems to be associated with smoking and drinking habits.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22295","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T14:43:50Z","timestamp":1591109030000},"page":"648-655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Abnormal cytology in oropharyngeal brushings and in oral rinses is not associated with HPV infection: The OHMAR study"],"prefix":"10.1002","volume":"128","author":[{"given":"Maria","family":"Benevolo","sequence":"first","affiliation":[{"name":"Pathology Department Regina Elena National Cancer Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Rollo","sequence":"additional","affiliation":[{"name":"Pathology Department Regina Elena National Cancer Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Giuliani","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infection\/HIV Unit San Gallicano Dermatological Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Pichi","sequence":"additional","affiliation":[{"name":"Otolaryngology Head Neck Surgery Department Regina Elena National Cancer Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Latini","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infection\/HIV Unit San Gallicano Dermatological Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul","family":"Pellini","sequence":"additional","affiliation":[{"name":"Otolaryngology Head Neck Surgery Department Regina Elena National Cancer Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Fenicia","family":"Vescio","sequence":"additional","affiliation":[{"name":"Infectious, Parasitic, and Immunomediated Disease Department Italian National Institute of Health (ISS) Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo","family":"Morrone","sequence":"additional","affiliation":[{"name":"Scientific Direction San Gallicano Dermatological Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Cristaudo","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infection\/HIV Unit San Gallicano Dermatological Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3250-1726","authenticated-orcid":false,"given":"Maria Gabriella","family":"Don\u00e0","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infection\/HIV Unit San Gallicano Dermatological Institute IRCCS Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12269"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2015.5504"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30716"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv403"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25585"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-19-0098"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.6995"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25810"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv302"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.05.025"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.75.0141"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32791"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.10508"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2019.07.008"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx535"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2017.12.008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djx005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-19-0185"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-11-0284"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28909"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184623"},{"volume-title":"International Agency for Research on Cancer (IARC) Monographs on the Evaluation of Carcinogenic Risks to Humans. Volume 100B\u2010Biological Agents. A Review of Human Carcinogens","year":"2012","author":"World Health Organization (WHO)","key":"e_1_2_8_23_1"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.5858\/2001-125-0127-UOBCCI","article-title":"Use of buccal cells collected in mouthwash as a source of DNA for clinical testing","volume":"125","author":"Heath EM","year":"2001","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_8_25_1","first-page":"687","article-title":"Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash","volume":"10","author":"Garcia\u2010Closas M","year":"2001","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2019.02.002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.101"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157976"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32713"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29660"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.4407"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djk179"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0347"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2013.50.3870"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2017.11.015"},{"volume-title":"Global Status Report on Alcohol and Health 2018","year":"2018","author":"World Health Organization (WHO)","key":"e_1_2_8_36_1"},{"volume-title":"WHO Global Report on Trends in Prevalence of Tobacco Smoking 2000\u20102025","year":"2018","author":"World Health Organization (WHO)","key":"e_1_2_8_37_1"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12981-018-0213-z"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.09.011"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2018.05.002"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcncy.22295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncy.22295","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T17:42:53Z","timestamp":1760290973000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.22295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,2]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/cncy.22295"],"URL":"https:\/\/doi.org\/10.1002\/cncy.22295","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"type":"print","value":"1934-662X"},{"type":"electronic","value":"1934-6638"}],"subject":[],"published":{"date-parts":[[2020,6,2]]},"assertion":[{"value":"2020-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}