{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:37:12Z","timestamp":1786977432200,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T00:00:00Z","timestamp":1624320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Testing for\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      gene alterations in patients with high\u2010grade serous carcinoma (HGSC) is a critical determinant of treatment eligibility for poly(adenosine diphosphate\u2010ribose) polymerase inhibitors in addition to providing vital information for genetic counselling. Many patients present with effusions necessitating therapeutic drainage, and this makes cytologic specimens (CySs) the initial diagnostic material for HGSC, often before histologic sampling. Initiating somatic\n                      <jats:italic>BRCA<\/jats:italic>\n                      testing on a CyS allows the\n                      <jats:italic>BRCA<\/jats:italic>\n                      status to be determined sooner, and this affects clinical management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Retrospectively, 8 cases of formalin\u2010fixed, paraffin\u2010embedded (FFPE) CySs of peritoneal or pleural fluid from patients with HGSC and known\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      alterations previously established by the testing of FFPE surgical specimens (SpSs) underwent next\u2010generation sequencing (NGS). Prospectively, 11 cases of peritoneal or pleural fluid from patients with HGSC but an unknown\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      status underwent NGS with fresh, alcohol\u2010fixed, and FFPE CySs, and they were compared with subsequent NGS on 4 SpSs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CySs yielded high\u2010quantity and high\u2010quality DNA for NGS analysis when sufficient tumor cellularity was present. Fresh, alcohol\u2010fixed, and FFPE CySs were all suitable for NGS and provided identical NGS results. SpS and CyS\n                      <jats:italic>BRCA<\/jats:italic>\n                      testing was concordant in 10 of 12 cases. The 2 discordant cases showed low tumor cellularity and quality in the CyS and the SpS, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Effusion CySs of HGSC are excellent sources for NGS testing for\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      genetic alterations when sufficient tumor cellularity is present. Fresh, alcohol\u2010fixed, and FFPE CySs are equivalent for NGS of\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      . NGS testing of HGSC CySs demonstrates good concordance with SpSs for the\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      status.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22484","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T18:42:27Z","timestamp":1624387347000},"page":"907-913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Validation of\n                    <i>BRCA<\/i>\n                    testing on cytologic samples of high\u2010grade serous carcinoma"],"prefix":"10.1002","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7351-0648","authenticated-orcid":false,"given":"Si Kei","family":"Lou","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Grenier","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program University Health Network University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Care","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Program University Health Network University of Toronto Toronto Ontario Canada"},{"name":"Department of Molecular Genetics University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanna","family":"McCuaig","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics University of Toronto Toronto Ontario Canada"},{"name":"Familial Cancer Clinic, Princess Margaret Hospital Cancer Centre University Health Network Toronto Ontario Canada"},{"name":"Lawrence S. Bloomberg Faculty of Nursing University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy L.","family":"Stockley","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaise","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology University of Toronto Toronto Ontario Canada"},{"name":"Laboratory Medicine Program University Health Network University of Toronto Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7507-2856","authenticated-orcid":false,"given":"Heather M.","family":"Ruff","sequence":"additional","affiliation":[{"name":"Department 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