{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:21Z","timestamp":1786977501160,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Liquid\u2010based cytology (LBC) is a widely used method for processing specimens obtained by endoscopic biopsy. This study evaluated next\u2010generation sequencing (NGS) analysis of LBC specimens to improve the diagnostic accuracy of pancreatic lesions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Upon the diagnosis of a suspected pancreatic mass, LBC residues were used retrospectively. The quantity and quality of DNA extracted from residual LBC samples were evaluated, and an NGS analysis targeting 6 genes (\n                      <jats:italic>KRAS<\/jats:italic>\n                      ,\n                      <jats:italic>GNAS<\/jats:italic>\n                      ,\n                      <jats:italic>TP53<\/jats:italic>\n                      ,\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      ,\n                      <jats:italic>SMAD4<\/jats:italic>\n                      , and\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      ) was performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The library was prepared from LBC specimens taken from 52 cases: 44 were successful, and 8 preparations failed. An analysis of DNA quantity and quality suggested that the success or failure of NGS implementation depended on both properties. The final diagnosis was achieved by a combination of the pathological analysis of the surgical excision or biopsy material with clinical information. Among the 33 cases of pancreatic ductal adenocarcinoma (PDAC),\n                      <jats:italic>KRAS<\/jats:italic>\n                      ,\n                      <jats:italic>TP53<\/jats:italic>\n                      ,\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      , and\n                      <jats:italic>SMAD4<\/jats:italic>\n                      mutations were identified in 31 (94%), 16 (48%), 3 (9%), and 2 (6%), respectively. Among the 11 benign cases, only a\n                      <jats:italic>KRAS<\/jats:italic>\n                      mutation was identified in 1 case. On the basis of NGS results, 18 of 33 PDACs (55%) were classified as highly dysplastic or more, and 10 of 11 benign lesions were evaluated as nonmalignant, which was consistent with the final diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>NGS analysis using LBC specimens from which DNA of appropriate quantity and quality has been extracted could contribute to improving the assessment of pancreatic tumor malignancies and the application of molecular\u2010targeted drugs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22525","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T07:11:34Z","timestamp":1634800294000},"page":"202-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation and diagnostic value of next\u2010generation sequencing analysis of residual liquid\u2010based cytology specimens of pancreatic masses"],"prefix":"10.1002","volume":"130","author":[{"given":"Yoko","family":"Sekita\u2010Hatakeyama","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Mitoro","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Sawai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Minami\u2010Nara General Medical Center Oyodo\u2010Cho Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroe","family":"Itami","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Uchiyama","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiko","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Sho","sequence":"additional","affiliation":[{"name":"Department of Surgery Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Yoshiji","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7729-1836","authenticated-orcid":false,"given":"Kinta","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"},{"name":"Department of Pathology National Cerebral and Cardiovascular Center Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-9508","authenticated-orcid":false,"given":"Chiho","family":"Ohbayashi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Nara Medical University Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,19]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Cancer Information Service. 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