{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:25:35Z","timestamp":1772007935653,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T00:00:00Z","timestamp":1636502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Human papillomavirus\u2013related oropharyngeal squamous cell carcinoma (HPV\u2010OPSCC) presents frequently as metastasis in a neck lymph node that may be cystic or necrotic. Fine\u2010needle aspiration (FNA) biopsies are often first\u2010line diagnostic procedures. p16 immunohistochemistry (IHC) is a surrogate marker for high\u2010risk HPV (hrHPV) infection but can be challenging to interpret. This study evaluated the use of hrHPV in situ hybridization (ISH) in cytology cell blocks of cystic neck lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four FNA cases with cell blocks and surgical correlates were evaluated. p16 IHC and hrHPV ISH were assessed on cell blocks (C\u2010p16 and C\u2010hrHPV ISH), and hrHPV ISH on surgical samples (S\u2010hrHPV ISH). All results were classified as negative, positive, or equivocal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two cases were excluded because of insufficient tissue on recut. On the basis of C\u2010hrHPV ISH cases, 12 were positive, 5 were negative, and 5 were equivocal. All 12 positive C\u2010hrHPV ISH cases had concordant S\u2010hrHPV ISH with no false positives. Of the 5 negative C\u2010hrHPV ISH cases, 4 had concordant S\u2010hrHPV ISH, and 1 had a discordant S\u2010hrHPV ISH. Of the 5 equivocal C\u2010hrHPV ISH cases, S\u2010hrHPV ISH were both positive and negative. Fourteen cases were equivocal by C\u2010p16; 9 cases were reliably classified by C\u2010hrHPV ISH (5 positive, 4 negative; 64%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>C\u2010hrHPV ISH can be reliably used, especially when positive. A negative or equivocal interpretation of C\u2010hrHPV ISH may warrant repeat testing. Compared to C\u2010p16, C\u2010hrHPV ISH is more frequently diagnostic and could be helpful for HPV\u2010OSCC diagnosis and management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22529","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T12:18:38Z","timestamp":1636546718000},"page":"154-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The utility of high\u2010risk human papillomavirus in situ hybridization in cytology cell block material from cystic head and neck lesions"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1839-9702","authenticated-orcid":false,"given":"Lucy M.","family":"Han","sequence":"first","affiliation":[{"name":"Department of Pathology University of California, San Francisco San Francisco California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara 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