{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:15:43Z","timestamp":1790572543220,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      The concept of mesothelioma in situ has been revisited and is a new World Health Organization diagnostic entity. The definition centers on ancillary techniques used in pleural mesothelioma (PM) assessment. At the authors' institution, most PM diagnoses are made on cytologic specimens. Effusion samples obtained before definitive PM diagnosis were interrogated using\n                      <jats:italic>BRCA1<\/jats:italic>\n                      \u2010\n                      <jats:italic>associated protein 1 gene<\/jats:italic>\n                      (\n                      <jats:italic>BAP1<\/jats:italic>\n                      ),\n                      <jats:italic>cyclin\u2010dependent kinase inhibitor 2A gene<\/jats:italic>\n                      (\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      ) and cytologic evaluation to assess whether early or possible in situ disease could be characterized.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      All cases of PM diagnosed between January 2008 and December 2019 were identified at a tertiary referral center. Patients who had a pleural fluid sample collected 24 months before the diagnosis were selected, numbering 8 in total. The cytomorphology of each sample was reviewed; and, retrospectively, BAP1 immunohistochemistry (IHC) and\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      fluorescence in situ hybridization (FISH) were performed on initial and diagnostic samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The initial samples were deemed benign in 5 cases and atypical mesothelial proliferations in 3 cases. A spectrum of apparently normal to atypical cytomorphologic changes was identified. BAP1 loss was present in 6 of 8 initial cases, whereas\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      homozygous deletion was identified in 1 of 7 initial cases. Either abnormality was identified in 7 of 8 initial samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Detectable abnormalities of BAP1 IHC and\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      FISH were present in pleural fluid specimens before the development of cytomorphologic features diagnostic of PM. This is the largest series to date describing cytology samples early in the course of PM development, thereby highlighting a possible cytological equivalent for mesothelioma in situ.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22548","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T10:09:44Z","timestamp":1644487784000},"page":"352-362","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Analysis of early pleural fluid samples in patients with mesothelioma: A case series exploration of morphology, BAP1, and\n                    <i>CDKN2A<\/i>\n                    status with implications for the concept of mesothelioma in situ in cytology"],"prefix":"10.1002","volume":"130","author":[{"given":"Amber","family":"Louw","sequence":"first","affiliation":[{"name":"Department of Anatomical Pathology PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"},{"name":"School of Medical and Health Sciences Edith Cowan University Perth Western Australia Australia"},{"name":"Institute for Respiratory Health Nedlands Western Australia Australia"},{"name":"National Center for Asbestos Related Diseases University of Western Australia Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"van Vliet","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Peverall","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Genomics PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"Colkers","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Acott","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenette","family":"Creaney","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health Nedlands Western Australia Australia"},{"name":"National Center for Asbestos Related Diseases University of Western Australia Nedlands Western Australia Australia"},{"name":"Department of Respiratory Medicine Sir Charles Gairdner Hospital Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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Gary","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute for Respiratory Health Nedlands Western Australia Australia"},{"name":"Department of Respiratory Medicine Sir Charles Gairdner Hospital Nedlands Western Australia Australia"},{"name":"School of Medicine University of Western Australia Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7941-5561","authenticated-orcid":false,"given":"Siaw Ming","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology PathWest Laboratory Medicine Queen Elizabeth II Medical Center Nedlands Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.086678"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050152"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-18-0804"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22798"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0365"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.855"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02223.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000471"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.65"},{"key":"e_1_2_8_11_1","first-page":"977","article-title":"BAP1 immunohistochemistry and p16 FISH to separate benign from malignant mesothelial proliferations","volume":"39","author":"Sheffield BS","year":"2015","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2016.10.010"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.09.026"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPP5R2ZJZKCLWN"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2008.45"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPT94JVWIHBKRD"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0240-SA"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2016.12.017"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12453"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23837"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2018.08.59"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000976"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21928"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0347-0"},{"key":"e_1_2_8_25_1","volume-title":"Thoracic Tumours [Internet]. 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