{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:32:12Z","timestamp":1787164332741,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>High\u2010risk human papilloma virus (HR HPV) testing and liquid\u2010based cytology are used for primary cervical screening. Digital cytology, based on whole\u2010slide scanned samples, is a promising technique for teaching and diagnostic purposes. The aim of our study was to evaluate the interobserver and intraobserver variation in low\u2010grade squamous lesions, HR HPV status bias, and the use of whole\u2010slide scanned digital cervical cytology slides.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifteen expert cytopathologists evaluated 71 digitalized ThinPrep slides (31 atypical squamous cells of undetermined significance [ASC\u2010US], 21 negative for intraepithelial lesion or malignancy, and 19 low\u2010grade squamous intraepithelial lesion cases). HR HPV data were accessible only in the second round.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In interobserver analysis, Kendall\u2019s coefficient of concordance was 0.52 in the first round and 0.58 in the second round. Fleiss\u2019 kappa values were 0.29 in the first round and 0.31 in the second round. In the ASC\u2010US category, Fleiss kappa increased from 0.19 to 0.22 in the second round and the increase was even higher expressed by Kendall\u2019s coefficient: from 0.42 to 0.52. In intraobserver analysis, personal scores were higher in the second round.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The interobserver and intraobserver variability in low\u2010grade squamous lesions was within fair agreement values in the present study, in line with previous works. The comparison of two rounds showed that expert cytopathologists are generally unbiased by the knowledge of HR HPV data, but that being informed of the HR HPV status leads to a better agreement. Stain quality and back discomfort were highlighted as factors affecting digital cytopathology use.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22624","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T08:42:15Z","timestamp":1657788135000},"page":"939-948","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Inter\u2010 and intraobserver agreement in whole\u2010slide digital ThinPrep samples of low\u2010grade squamous lesions of the cervix uteri with known high\u2010risk HPV status: A multicentric international study"],"prefix":"10.1002","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4089-0450","authenticated-orcid":false,"given":"Ivana","family":"Kholov\u00e1","sequence":"first","affiliation":[{"name":"Tampere University Faculty of Medicine and Health Technology  Tampere Finland"},{"name":"Pathology, Fimlab Laboratories  Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9194-8782","authenticated-orcid":false,"given":"Giovanni","family":"Negri","sequence":"additional","affiliation":[{"name":"Pathology Unit, Central Hospital Bolzano  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Nasioutziki","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Aristotle University of Thessaloniki and Molecular Cytopathology Department  Hippokration General Hospital Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ventura","sequence":"additional","affiliation":[{"name":"Department of Statistical Sciences University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arrigo","family":"Capitanio","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital Link\u00f6ping  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9846-6682","authenticated-orcid":false,"given":"Massimo","family":"Bongiovanni","sequence":"additional","affiliation":[{"name":"Synlab Pathology  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Cross","sequence":"additional","affiliation":[{"name":"South of Tyne Pathology Department, Queen Elizabeth Hospital  Gateshead England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Bourgain","sequence":"additional","affiliation":[{"name":"Imelda Hospital, Bonheiden  Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Edvardsson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cytology Karolinska University Laboratory  Karolinska Hospital Huddinge Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9482-622X","authenticated-orcid":false,"given":"Rosario","family":"Granados","sequence":"additional","affiliation":[{"name":"University Hospital de Getafe  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Lipi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Pathology and Oncological Cytology Wroclaw Medical University  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen Christina","family":"Obermann","sequence":"additional","affiliation":[{"name":"University Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Pinamonti","sequence":"additional","affiliation":[{"name":"University Hospital Cattinara  Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrieta","family":"Sidlova","sequence":"additional","affiliation":[{"name":"St. Elisabeth Cancer Institute Hospital  Bratislava Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margareta","family":"Strojan Fle\u017ear","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Ljubljana Institute of Pathology  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Folkert J.","family":"van Kemenade","sequence":"additional","affiliation":[{"name":"Erasmus MC University Medical Center  Department of Pathology Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danijela","family":"Vrdoljak\u2010Mozetic","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cytology, Clinical Hospital Centre Rijeka  Rijeka Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5737-2249","authenticated-orcid":false,"given":"Ambrogio","family":"Fassina","sequence":"additional","affiliation":[{"name":"Department of Medicine (DIMED), Surgical Pathology &amp; 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