{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:53:11Z","timestamp":1773251591815,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>High\u2010risk human papillomavirus (HR\u2010HPV) status is critical for the diagnosis, prognosis, and treatment of patients with oropharyngeal squamous cell carcinoma (OPSCC). Patients often present with enlarged cervical nodes, and fine\u2010needle aspiration cytology (FNAC) is frequently the initial diagnostic procedure. Although p16 is the most widely used surrogate marker, problems with interpretation can limit its utility in FNAC. HR\u2010HPV RNA in situ hybridization (ISH) has emerged as a specific way to assess HPV status on cell block preparations of cervical nodes. The authors evaluated the utility of HR\u2010HPV ISH in conventional smears and liquid\u2010based cytology (LBC) preparations of metastatic head and neck squamous cell carcinoma (SCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010one aspirates of proven, HPV\u2010related SCC (confirmed by p16 and\/or HR\u2010HPV ISH in corresponding surgical specimens) were selected. Ten aspirates of HPV\u2010negative SCC were also retrieved. HR\u2010HPV ISH was performed on 27 smears and 14 LBC preparations. All results were scored as positive, equivocal, or negative.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighty\u2010four percent of metastatic, HPV\u2010related SCCs were positive for HR\u2010HPV RNA ISH, with high number of signals (<jats:italic>n<\/jats:italic>\u00a0=\u00a019) and low number of signals (<jats:italic>n<\/jats:italic>\u00a0=\u00a07), whereas five HPV\u2010related SCCs were equivocal. All metastatic, HPV\u2010negative SCCs were negative for HR\u2010HPV ISH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HR\u2010HPV ISH can be reliably performed on smears or LBC preparations, particularly when cell blocks are unavailable or paucicellular. Results were easy to interpret when high numbers of signals were present but were challenging in aspirates with low or rare number of signals. The current study suggests that HR\u2010HPV ISH could be used as the initial testing modality for determining HPV status in FNAC specimens of metastatic SCC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22659","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T10:55:41Z","timestamp":1667991341000},"page":"165-170","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Utility of high\u2010risk HPV RNA chromogenic in situ hybridization in cytology smears and liquid\u2010based preparations from metastatic head and neck squamous cell carcinoma"],"prefix":"10.1002","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8385-2748","authenticated-orcid":false,"given":"Jaylou M.","family":"Velez Torres","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Turky","family":"Alkathery","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Pathology and Laboratory Medicine Jackson Memorial Hospital  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8665-312X","authenticated-orcid":false,"given":"Youley","family":"Tjendra","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqin","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-2044","authenticated-orcid":false,"given":"Darcy A.","family":"Kerr","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Dartmouth\u2010Hitchcock Medical Center  Lebanon New Hampshire USA"},{"name":"Geisel School of Medicine at Dartmouth  Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Gomez\u2010Fernandez","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22178"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24593"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22529"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.ccr\u201006\u20101690"},{"issue":"1","key":"e_1_2_8_6_1","first-page":"76","article-title":"Fine needle aspiration biopsy in the diagnosis of lymphadenopathy in 1,103 patients. 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