{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:38:23Z","timestamp":1770824303447,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fine\u2010needle aspiration is used as a diagnostic tool in head and neck oropharyngeal squamous cell carcinoma and its metastases. Prognosis and treatment rely on the presence or absence of the human papilloma virus. The purpose of this study was to validate the performance of the Aptima HPV assay using Hema\u2010Diff stained fine\u2010needle aspiration smears in the diagnosis of human papilloma virus\u2010related oropharyngeal squamous cell carcinoma using a simplified method to obtain tumor cells for testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with a diagnosis of squamous cell carcinoma and positive p16 immunohistochemical staining were identified. Aptima Specimen Transport Media was used to remove tumor cells from the Hema\u2010Diff stained slides using a moistened swab. The selected cells were tested for high risk\u2010human papilloma virus using the Aptima HPV assay and Aptima HPV 16 18\/45 genotype assay. The results were compared with the p16 immunohistochemical staining of the related cell block and surgical specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010one of the 21 (100%) p16\u2010positive cases were found to be positive for high risk\u2010human papilloma virus, whereas 20 of 21 (95%) negative cases were found to be negative for high risk\u2010human papilloma virus using the Aptima HPV assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Aptima HPV assay can be used to detect high\u2010risk human papilloma virus in Hema\u2010Diff stained fine\u2010needle aspiration smears of oropharyngeal squamous cell carcinoma with a sensitivity of 100% and a specificity of 95%. This provides a valuable alternative to p16 immunohistochemical staining of cell block sections that often lack appropriate numbers of tumor cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22662","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T10:09:19Z","timestamp":1666778959000},"page":"171-178","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A simplified molecular method to detect high\u2010risk HPV using the Aptima HPV assay on head and neck FNA smears"],"prefix":"10.1002","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-9285","authenticated-orcid":false,"given":"Joseph P.","family":"Gormley","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin School of Medicine and Public Health  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne 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