{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T06:52:05Z","timestamp":1786171925357,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T00:00:00Z","timestamp":1668729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>High\u2010grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of this study was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and <jats:italic>BRCA1\/2<\/jats:italic> mutations from ascites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 45 patients with histologically confirmed primary HGSC and malignant ascites. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki\u201067 was performed on cytospins and cytoblocks prepared from ascites. Next\u2010generation sequencing (NGS) was used to detect germline\/somatic <jats:italic>BRCA1\/2<\/jats:italic> mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of primary tumor. Cronbach \u03b1 and \u03c7<jats:sup>2<\/jats:sup> statistics, respectively, were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ICC\/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (\u03b1\u00a0&gt;\u00a00.75), whereas poor reliability and significant differences were observed for Ki\u201067 between ascites and tissue blocks (\u03b1\u00a0&lt;\u00a00.26; <jats:italic>p<\/jats:italic> &lt; .001 [Kruskal\u2013Wallis]). For germline <jats:italic>BRCA1\/2<\/jats:italic> mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrate that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline <jats:italic>BRCA1\/2<\/jats:italic> mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic <jats:italic>BRCA1\/2<\/jats:italic> mutations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22664","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T12:33:56Z","timestamp":1668774836000},"page":"188-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cytopathological assessment is an accurate method for identifying immunophenotypic features and <i>BRCA1\/2<\/i> mutations of high\u2010grade serous carcinoma from ascites"],"prefix":"10.1002","volume":"131","author":[{"given":"Simona","family":"Miceska","sequence":"first","affiliation":[{"name":"Institute of Oncology Ljubljana  Ljubljana Slovenia"},{"name":"Faculty of Medicine University of Ljubljana  Ljubljana 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Kuzmanov","sequence":"additional","affiliation":[{"name":"Institute of Oncology Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0160pela","family":"Smrkolj","sequence":"additional","affiliation":[{"name":"University Medical Centre  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branko","family":"Cvjeti\u010danin","sequence":"additional","affiliation":[{"name":"University Medical Centre  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Bebar","sequence":"additional","affiliation":[{"name":"Institute of Oncology Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Globo\u010dnik Kukovica","sequence":"additional","affiliation":[{"name":"Institute of Oncology Ljubljana  Ljubljana 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