{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T17:43:49Z","timestamp":1760291029786,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fine\u2010needle aspiration cytology (FNAC) is the first\u2010line diagnostic procedure for salivary gland masses. Secretory carcinoma (SC) is characterized by <jats:italic>ETV6<\/jats:italic> and <jats:italic>RET<\/jats:italic> rearrangements detected by fluorescence in situ hybridization (FISH) or reverse transcriptase\u2010polymerase chain reaction optimized for paraffin\u2010embedded and fresh\u2010frozen tissue, respectively. The authors performed FISH on cytological material to assess its role in the diagnosis of SC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>FNACs with SC as a diagnostic consideration and cases diagnosed as SC on histology with a corresponding FNAC with any diagnosis were evaluated for <jats:italic>ETV6<\/jats:italic> rearrangement by FISH. If acinic cell carcinoma (ACC) was a differential diagnosis and <jats:italic>ETV6<\/jats:italic> rearrangement was absent, <jats:italic>NR4A3<\/jats:italic> FISH was performed. FISH results were compared with those on histological specimens, where available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifteen cases were included. FISH initially performed on three cell blocks did not yield good results, was then performed on direct smears, and was interpretable in 14 cases (93.3%). An <jats:italic>ETV6<\/jats:italic> rearrangement was identified in seven cases (50%), and an <jats:italic>NR4A3<\/jats:italic> rearrangement was identified in three cases (21.4%), providing a confirmatory diagnosis in 10 of 15 cases (66.7%). The Milan System for Reporting Salivary Gland Cytopathology category was altered in two cases (6.7%). Complete correlation (100%) was seen with FISH on corresponding histological specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>With minor modifications, the FISH procedure can be optimized for FNAC smears with results comparable to those on histological specimens. <jats:italic>ETV6<\/jats:italic> FISH testing on cytological smears in cases suspected as SC improves the diagnostic accuracy of FNAC and can help lower the proportion of the Milan categories <jats:italic>salivary gland neoplasm of uncertain malignant potential<\/jats:italic> and <jats:italic>suspicious for malignancy<\/jats:italic>, maximizing diagnostic information from less invasive samples and aiding in patient management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22699","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T01:06:11Z","timestamp":1679965571000},"page":"442-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fluorescence in situ hybridization for <i>ETV6<\/i> gene rearrangements on destained cytological smears: Role in diagnosis of secretory carcinoma of salivary gland"],"prefix":"10.1002","volume":"131","author":[{"given":"Ria","family":"Mahendru","sequence":"first","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chetna","family":"Sarma","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aanchal","family":"Kakkar","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head Neck Surgery All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavneet","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alok","family":"Thakar","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head Neck Surgery All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10006\u2010011\u20100291\u20108"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100714.2011.01069.x"},{"key":"e_1_2_9_4_1","first-page":"681","article-title":"Diagnostic value of fine\u2010needle aspiration from parotid gland lesions","volume":"4","author":"Lurie M","year":"2002","journal-title":"Isr Med Assoc J"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000497109"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014\u20100167\u2010ra"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0297\u2010170"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.Ccr\u201011\u20101870"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1083"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0b013e3181de3021"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0b013e3181d9efcc"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.09.012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000392"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000972"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001065"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001535"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102559.2012.04232.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25536"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2019.01.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201001970\u20104"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.25057"},{"volume-title":"NCCN Guidelines\u00ae. 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