{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:59:16Z","timestamp":1787839156832,"version":"build-2784847793"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnosis of salivary gland neoplasms is challenging, especially on cytological specimens acquired by fine\u2010needle aspiration. The recently implemented standardized Milan system for reporting salivary gland cytopathology provides an estimated risk of malignancy (ROM); yet, for two of the categories, the diagnosis of the lesion remains unclear. However, a precise diagnosis is desirable for optimal patient management, including planning of surgery and imaging procedures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cytological specimens (<jats:italic>n<\/jats:italic>\u00a0=\u00a0106) were subjected to molecular analysis using the SalvGlandDx panel. The risk of malignancy was calculated for each detected alteration based on the diagnosis of the resection specimen. By taking into account the molecular alterations, their associated ROM, the clinical and cytological features, and the current literature, the Milan category was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of <jats:italic>n<\/jats:italic>\u00a0=\u00a063 technically valid cases, 76% revealed a molecular alteration. A total of 94% of these molecularly altered cases could be assigned to a different Milan category when additionally taking molecular results into account. In only 2% of the salivary gland neoplasms of uncertain malignant potential, in which a molecular alteration was detected, the classification remained salivary gland neoplasms of uncertain malignant potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Molecular analysis of cytological specimens provides a benefit in classifying salivary gland neoplasms on fine\u2010needle aspiration. It can improve the ROM estimation and thus help to assign cases of formerly unknown malignant potential to clearly benign or malignant categories.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22814","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T12:24:43Z","timestamp":1712060683000},"page":"435-446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Molecular analysis using SalvGlandDx improves risk of malignancy estimation and diagnosis of salivary gland cytopathology: An exploratory multicenter study"],"prefix":"10.1002","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6874-9649","authenticated-orcid":false,"given":"Sandra N.","family":"Freiberger","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Ikenberg","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demi","family":"van Egmond","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Claerhout","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University Hospitals Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"van Wezel","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle Vanden","family":"Bempt","sequence":"additional","affiliation":[{"name":"Department of Human Genetics University Hospitals Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen N.","family":"van Rossem","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon A.","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M","family":"Clement","sequence":"additional","affiliation":[{"name":"Department of Oncology Section Experimental Oncology Leuven Cancer Institute KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent Vander","family":"Poorten","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology\u2010Head and Neck Surgery University Hospitals Leuven Leuven Cancer Institute  Leuven Belgium"},{"name":"Department of Oncology Section Head and Neck Oncology Leuven Cancer Institute KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Pathology Leiden University Medical Center  Leiden Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Hauben","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospitals Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels J.","family":"Rupp","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2021.03.008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2022\u20100529\u2010RA"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24515"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22435"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.5914"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22312"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22678"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000980"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010022\u201001420\u20101"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2020.01.019"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23009"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001178\u2010z"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22756"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22762"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2023.05.001"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.15074"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14817"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/dad.0000000000002276"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.21965"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010021\u201001297\u20106"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000669"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010016\u20100730\u20109"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.12.016"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2017.11.011"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001258"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100576\u20102"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/pap.0000000000000362"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010022\u201001513\u2010x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.13166"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001807"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001279"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.30179"},{"key":"e_1_2_10_34_1","volume-title":"WHO Classification of Head and Neck Tumours","author":"El\u2010Naggar A","year":"2017"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14840"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13363"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.22814","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:56:19Z","timestamp":1760266579000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.22814"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,2]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/cncy.22814"],"URL":"https:\/\/doi.org\/10.1002\/cncy.22814","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,2]]},"assertion":[{"value":"2024-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}