{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T23:36:16Z","timestamp":1789688176027,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,16]],"date-time":"2024-06-16T00:00:00Z","timestamp":1718496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      A cytologic diagnosis of\n                      <jats:italic>atypical squamous cells, cannot exclude high\u2010grade squamous lesion<\/jats:italic>\n                      (ASC\u2010H) poses a disproportionately high risk of cervical cancer development. The objective of this study was to analyze type\u2010specific risks by mapping human papillomavirus (HPV) genotypes in ASC\u2010H cytology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In total, 1,048,581 Papanicolaou tests that had ASC\u2010H cytology were retrieved. Concurrent HPV genotyping using proprietary multiplex real\u2010time (MRT) and polymerase chain reaction (PCR) HPV tests and histologic follow\u2010up findings were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among 1678 patients who had ASC\u2010H findings (0.16%), 1414 (84.3%) underwent concurrent HPV genotyping (MRT, 857; HPV PCR test, 557). The overall high\u2010risk HPV (hrHPV)\u2010positive rate was 84.4%. Of the 857 MRT cases, 63.9% were infected with a single hrHPV, and 24.4% had multiple genotypes. The most prevalent HPV types were HPV16\/52\/58\/33\/31. Lesions that were identified as cervical intraepithelial neoplasia 2 or worse (CIN2+) were detected in 498 of 906 cases (55.0%), including 81 cervical carcinomas (8.9%). The risk of CIN2+ for the composite group of HPV16\/52\/58\/33\/31\u2010positive cases was 62.7%, representing 90.7% (264 of 291) of total CIN2+ lesions in ASC\u2010H\/hrHPV\u2013positive cases by MRT. CIN2+ lesions were detected in 108 of 142 (76.1%) HPV16\u2010positive and\/or HPV18\u2010positive women by the PCR the HPV test. Among 128 hrHPV\u2010negative ASC\u2010H cases by both methods, CIN2+ lesions were identified in 21 of 128 (16.4%), including five cervical carcinomas (3.9%). The sensitivity, specificity, positive predictive value, and negative predictive value for patients in the composite group with HPV16\/52\/58\/33\/31 were 88.0%, 40.8%, 62.7%, and 75.0%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Papanicolaou tests classified as ASC\u2010H are associated with a high CIN2+ rate and warrant colposcopy, regardless of HPV status. The extent to which the risk\u2010stratification provided by comprehensive HPV genotyping can inform the management of ASC\u2010H cytology remains to be explored.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.22877","type":"journal-article","created":{"date-parts":[[2024,6,16]],"date-time":"2024-06-16T11:00:20Z","timestamp":1718535620000},"page":"588-598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genotype profile of HPV in ASC\u2010H cytology and histologic follow\u2010up\u2014prevalence, distribution, and risk: A retrospective study of 1414 cases"],"prefix":"10.1002","volume":"132","author":[{"given":"Yihua","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2983-7168","authenticated-orcid":false,"given":"Tiannan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Starr","sequence":"additional","affiliation":[{"name":"Department of Pathology Magee\u2010Womens Hospital of University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianxu","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pathology The Third Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianrong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1052-118X","authenticated-orcid":false,"given":"Xiang","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Pathology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7524-3048","authenticated-orcid":false,"given":"Chengquan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology Magee\u2010Womens Hospital of University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.86"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.10400"},{"key":"e_1_2_7_4_1","volume-title":"WHO Guideline for Screening and Treatment of Cervical Pre\u2010Cancer Lesions for Cervical Cancer Prevention","author":"World Health Organization (WHO)","year":"2021"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21628"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1470\u20102045(11)70188\u20107"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055\u20109965.epi\u201013\u20100173"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2014.11.076"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000442407"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443679"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2017.03.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.5858\/2008\u2010132\u20101290\u2010hptarr"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014\u20100393\u2010cp"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22333"},{"key":"e_1_2_7_15_1","unstructured":"College of American Pathologists (CAP).CAP Cytopathology Checklist. 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