{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:12:20Z","timestamp":1789866740666,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Trichorhinophalangeal syndrome 1 (TRPS1) expression in primary breast and other solid tumors has been investigated but its role as a marker in metastatic tumors is unclear. The objective of this study was to evaluate the sensitivity and specificity of TRPS1 as a breast cancer immunomarker in metastatic tumors that originated from breast, M\u00fcllerian, lung, gastrointestinal (GI), and pancreatic primary tumors with cell blocks from fine\u2010needle aspiration (FNA) and effusion specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell blocks were immunostained with anti\u2010TRPS1 monoclonal antibody (clone EPR16171). Histochemical scores (H scores) (proportion \u00d7 intensity; range, 0\u2013300) were assigned; H scores of \u226510 were considered positive. Overall, 160 specimens were examined, including 127 FNAs (35 breast, 25 M\u00fcllerian, 36 lung, and 31 GI and pancreatic carcinomas) and 33 effusion specimens (18 breast, 12 M\u00fcllerian, one lung, and two GI carcinomas).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TRPS1 was positive in 51 of 53 (96%) metastatic breast carcinomas and in 28 of 107 (26.2%) nonbreast metastatic tumors. Metastatic breast carcinoma showed the highest mean H score of 247.35, compared to 45.36 in M\u00fcllerian, 8.4 in lung, and 5.88 in GI tumors. The sensitivity and specificity of TRPS1 for identifying a breast origin in metastatic tumors was 96.22% and 72.89%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite high overall sensitivity, TRPS1 showed lower specificity as a breast immunomarker because of its expression in nonbreast tumors. The mean H score in nonbreast tumors was significantly lower than in metastatic breast tumors. It is important to recognize the broad range of expression of TRPS1 in metastatic breast and nonbreast tumors to avoid an incorrect determination of a metastatic tumor\u2019s organ of origin.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22901","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T12:49:14Z","timestamp":1726145354000},"page":"799-808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Trichorhinophalangeal syndrome 1 expression in breast and nonbreast metastases from M\u00fcllerian, lung, gastrointestinal tract, and pancreatic primary tumors by immunohistochemistry with cytology cell block specimens"],"prefix":"10.1002","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-6616","authenticated-orcid":false,"given":"Deepak","family":"Donthi","sequence":"first","affiliation":[{"name":"Department of Anatomical Pathology The University of Texas MD Anderson 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