{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:47:50Z","timestamp":1792223270781,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:00:00Z","timestamp":1735776000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>This study aimed to assess the feasibility of implementing the Idylla system, an ultra\u2010rapid, cartridge\u2010based assay, as an extension of rapid on\u2010site evaluation (ROSE) in cytology. The authors conducted a pilot validation study on specimens from non\u2013small cell lung carcinoma, thyroid carcinoma, and melanoma, evaluating four assays designed to detect alterations in <jats:italic>KRAS<\/jats:italic>, <jats:italic>EGFR<\/jats:italic>, <jats:italic>BRAF<\/jats:italic>, gene fusions, and expression imbalances in <jats:italic>ALK<\/jats:italic>, <jats:italic>ROS1<\/jats:italic>, <jats:italic>RET<\/jats:italic>, <jats:italic>NTRK<\/jats:italic>1\/2\/3, and <jats:italic>MET<\/jats:italic> exon 14 skipping transcripts. They investigated the feasibility of providing accurate biomarker molecular testing results in a cytopathology laboratory within hours of specimen collection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The authors evaluated the performance characteristics and turn\u2010around\u2010time of the Idylla system by testing a total of 144 cartridge assays across various specimen types, including fine\u2010needle aspirate smears, formalin\u2010fixed paraffin\u2010embedded (FFPE) cell blocks, small tissue biopsy FFPE blocks, and control cell line FFPE scrolls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average time from specimen input to results output was 2\u20133 hours. Accuracy across the four cartridge types was: <jats:italic>KRAS<\/jats:italic> assay: 100%, <jats:italic>EGFR<\/jats:italic> assay: 94%, <jats:italic>BRAF<\/jats:italic> assay: 100%, and GeneFusion assay: 94%. Analytical sensitivity ranged from 1% to 5% variant allele frequency for all assays. Inter\u2010assay precision and analytical specificity were both 100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Using the Idylla system, actionable genetic alterations can be reliably detected within 2\u20133 hours from cytology and small biopsy samples with minimal input requirements. The findings of this study demonstrate the feasibility of incorporating same\u2010day molecular testing as part of ROSE procedures in the cytopathology laboratory, ultimately shortening the time from procedure to personalized treatment for cancer patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.22930","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T22:44:04Z","timestamp":1735857844000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Same\u2010day molecular testing for targetable mutations in solid tumor cytopathology\u2014The next frontier of the rapid on\u2010site evaluation"],"prefix":"10.1002","volume":"133","author":[{"given":"J. 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