{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T01:01:32Z","timestamp":1775696492356,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T00:00:00Z","timestamp":1740355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>The aim of this project is to assess interobserver agreement for programmed death\u2010ligand 1 (PD\u2010L1) scoring on of non\u2013small cell lung cancer (NSCLC) on cytological specimens in a large\u2010scale multicenter study, by exploiting the cell block\u2010derived tissue microarray (cbTMA) approach.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 65 cell blocks (CB) diagnosed as NSCLC were retrospectively collected and selected for TMA preparation. Hematoxylin\u2013eosin and PD\u2010L1 stained slides were digitized and uploaded on a free web sharing platform. Participants were asked to provide PD\u2010L1 assessment by using the clinically relevant cutoff of tumor proportion score (TPS) (<1%; 1%\u201349%; >50%). Interobserver agreement was calculated using Fleiss\u2019s \u03ba.<\/jats:p><\/jats:sec>Results<\/jats:title>Of 65 CBs, 11 were deemed not suitable; therefore, an overall number of 54 cores were used for the preparation of four TMAs. A total of 1674 evaluations were provided by 31 cytopathologists from 21 different institutions in nine countries. The statistical analysis showed a moderate overall agreement (\u03ba\u00a0=\u00a00.49). The highest agreement was achieved in the TPS >50% category (\u03ba\u00a0=\u00a00.57); moderate agreement was observed in TPS <1% category (\u03ba\u00a0=\u00a00.51) and the lowest \u03ba value was obtained for TPS 1%\u201349% category (k\u00a0=\u00a00.32).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The overall moderate agreement observed showed that there is still room for improvement in inter\u2010pathologist agreement for PD\u2010L1 evaluation on cytological samples, highlighting the need for standardization in sample preparation, focused training in PD\u2010L1 evaluation on cytological material, and the integration of machine learning tools to improve interobserver consistency.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncy.70003","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T11:46:24Z","timestamp":1740570384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["InterobServer AgreeMent in Pd\u2010l1 evaLuatIoN on cytoloGical samples\u2014SAMPLING project: A multi\u2010institutional, international study"],"prefix":"10.1002","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0803-0293","authenticated-orcid":false,"given":"Gennaro","family":"Acanfora","sequence":"first","affiliation":[{"name":"Department of Public Health University of Naples \u201cFederico II\u201d Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonino","family":"Iaccarino","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples \u201cFederico II\u201d Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"Cerbelli","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnology Polo Pontino Sapienza University Roma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Di Cristofano","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnology Polo Pontino Sapienza University Roma Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Bellevicine","sequence":"additional","affiliation":[{"name":"Department of Public Health University of Naples \u201cFederico II\u201d Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Barberis","sequence":"additional","affiliation":[{"name":"Advanced Molecular Diagnostics Istituto Europeo di Oncologia IRCCS Milano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Bonoldi","sequence":"additional","affiliation":[{"name":"Niguarda Cancer Center Niguarda Hospital Milano Milano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5970-1803","authenticated-orcid":false,"given":"Lukas","family":"Bubendorf","sequence":"additional","affiliation":[{"name":"Institute of Medical Genetics and Pathology University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Calaminus","sequence":"additional","affiliation":[{"name":"Institut fur Pathologie Uniklinik Koln Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Severo","family":"Campione","sequence":"additional","affiliation":[{"name":"Department of Advanced Diagnostic\u2010Therapeutic Technologies and Health Services Section of Anatomic Pathology A. 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