{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:16:37Z","timestamp":1787505397204,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010040","name":"Taishan Scholar Project of Shandong Province","doi-asserted-by":"publisher","award":["tsqn202211376"],"award-info":[{"award-number":["tsqn202211376"]}],"id":[{"id":"10.13039\/501100010040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172616"],"award-info":[{"award-number":["82172616"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022MH241"],"award-info":[{"award-number":["ZR2022MH241"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2021MH097"],"award-info":[{"award-number":["ZR2021MH097"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018904","name":"Beijing Xisike Clinical Oncology Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018904","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n This study aimed to investigate the prevalence and cytological features of\n Dicer 1<\/jats:italic>\n ,\n Ribonuclease III<\/jats:italic>\n (\n DICER<\/jats:italic>\n )\u2010mutant fine\u2010needle aspiration (FNA) specimens in thyroid nodules classified as Bethesda II, III, and IV categories. The authors also sought to explore the relationship between\n DICER1<\/jats:italic>\n and\n BRAF<\/jats:italic>\n V600E<\/jats:sup>\n mutations in Bethesda III thyroid nodules.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The authors collected a series of consecutive FNA cases diagnosed as Bethesda category II, III, and IV from a medical center over the course of 1 year.\n DICER1<\/jats:italic>\n exons 24 and 25 and\n TERT<\/jats:italic>\n promoter mutations were detected by Sanger sequencing in all cases, and\n BRAF<\/jats:italic>\n V600E<\/jats:sup>\n mutations were detected by amplification refractory mutation system PCR in Bethesda III and IV cases.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A total of 899 patients were included in the study.\n DICER1<\/jats:italic>\n mutations were identified in 52 patients: 20 in Bethesda category II (6.2%, 20 of 322), 25 in Bethesda category III (4.9%, 25 of 510), and seven in Bethesda category IV (10.4%, 7 of 67). Among the 510 Bethesda III FNA samples, 76 harbored the\n BRAF<\/jats:italic>\n V600E<\/jats:sup>\n mutation and 25 harbored\n DICER1<\/jats:italic>\n mutations.\n BRAF<\/jats:italic>\n V600E<\/jats:sup>\n and\n DICER1<\/jats:italic>\n mutations were mutually exclusive. In Bethesda category II and III cases, patients with\n DICER1<\/jats:italic>\n \u2010mutant nodules were younger and had larger nodule volumes compared to those without\n DICER1<\/jats:italic>\n mutations. All\n DICER1<\/jats:italic>\n \u2010mutant Bethesda III FNAs were\u00a0classified as\u00a0atypia of undetermined significance (AUS)\u2010other.\n TERT<\/jats:italic>\n promoter mutations (c. \u2010118G>T and c. \u2010144 C>T) were identified in two FNA samples.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The findings of this study suggest that\n DICER1<\/jats:italic>\n \u2010mutant nodules are unlikely to be\n BRAF<\/jats:italic>\n \u2010mutant carcinomas. Further study of the molecular characteristics of\n DICER1<\/jats:italic>\n \u2010mutant FNAs will contribute to more accurate cytological diagnosis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/cncy.70029","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T10:49:06Z","timestamp":1751539746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["DICER1<\/i>\n mutations in Bethesda category II, III, and IV thyroid nodules: A mutually exclusive relationship with\n BRAF<\/i>\n V600E<\/sup>\n mutation"],"prefix":"10.1002","volume":"133","author":[{"given":"Yulian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology School of Basic Medicine Qingdao University Qingdao Shandong China"},{"name":"Department of Pathology Jining No.1 People\u2019s Hospital Jining Shandong 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzhu","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Pathology The Affiliated Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology The Affiliated Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-0232","authenticated-orcid":false,"given":"Jigang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology The Affiliated Hospital of Qingdao University Qingdao Shandong 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