{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:56:23Z","timestamp":1773014183821,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Thyroid adenoma\u2010associated (\n                      <jats:italic>THADA<\/jats:italic>\n                      ) gene fusions are recurrent alterations in thyroid nodules that lead to the overexpression of insulin\u2010like growth factor messenger RNA\u2013binding protein 3 (IMP3). Although IMP3 immunohistochemistry has been studied in thyroid tumors, its utility for detecting\n                      <jats:italic>THADA<\/jats:italic>\n                      fusion\u2013positive thyroid specimens remain unassessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The authors evaluated IMP3 expression in cytologic and surgical specimens from 20 indeterminate thyroid nodules. Eleven cases harbored\n                      <jats:italic>THADA<\/jats:italic>\n                      fusions, including\n                      <jats:italic>THADA::IGF2BP3<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a09) and\n                      <jats:italic>THADA::TRA2A<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a02), whereas nine control cases lacked detectable\n                      <jats:italic>THADA<\/jats:italic>\n                      fusions. Hematoxylin\u2010and\u2010eosin staining and immunohistochemistry for IMP3 and NRAS Q61R were performed on available cell blocks and surgical specimens. Staining was scored based on extent and intensity, with a composite score &gt;2 considered positive.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All nodules with\n                      <jats:italic>THADA<\/jats:italic>\n                      fusions exhibited strong, diffuse, cytoplasmic IMP3 staining in lesional follicular cells, with no staining in adjacent normal thyroid tissue. All control cases were negative for IMP3. NRAS Q61R immunostaining was negative across all cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The current findings in a limited cohort demonstrate that IMP3 is a promising surrogate marker for indeterminate thyroid nodules with\n                      <jats:italic>THADA<\/jats:italic>\n                      fusions in both cytologic and surgical specimens. In addition, IMP3 staining may aid in detecting subtle capsular or vascular invasion.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70076","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:13:25Z","timestamp":1772990005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Testing insulin\u2010like growth factor messenger RNA\u2013binding protein 3 as a surrogate immunohistochemical marker for indeterminate thyroid nodules with THADA fusion in both cytologic and surgical specimens"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-4582","authenticated-orcid":false,"given":"Di","family":"Ai","sequence":"first","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Steward\u2010Tharp","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine School of Medicine Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2772-6077","authenticated-orcid":false,"given":"Wen\u2010Yu","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine School of Medicine Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0224-3167","authenticated-orcid":false,"given":"Qiuying","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine School of Medicine Oregon Health Science University  Portland Oregon 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