{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T10:06:40Z","timestamp":1779012400525,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      testing is currently recommended at diagnosis for high\u2010grade serous ovarian carcinoma (HGSOC) because of its impact on patients' survival when treated with poly(adenosine diphosphate ribose) polymerase inhibitors. Standard clinical practice involves analyzing\n                      <jats:italic>BRCA1<\/jats:italic>\n                      \/\n                      <jats:italic>2<\/jats:italic>\n                      genes in formalin\u2010fixed, paraffin\u2010embedded (FFPE) histological specimens. However, because neoplastic ascites is a common clinical presentation in HGSOC and provides a good source of neoplastic cells via a less invasive procedure, it is worthwhile to explore the feasibility of\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      testing on cytological specimens obtained from malignant ascites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      status was analyzed in 34 ascites\u2010derived cytological samples via an amplicon\u2010based next\u2010generation sequencing (NGS) approach, and the results were compared with those from FFPE tissues previously tested in routine clinical practice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A perfect match was observed between\n                      <jats:italic>BRCA1<\/jats:italic>\n                      \/\n                      <jats:italic>2<\/jats:italic>\n                      testing results from neoplastic ascites and surgical samples (100% concordance) for all pathogenic variants, including both germline and somatic mutations. This is the first study to report such high concordance within the largest collection of somatic variants analyzed to date. Additionally, molecular NGS testing was demonstrated to be feasible even in malignant ascites with a low tumor fraction and with archived material.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study shows that ascites can be a suitable specimen for\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      NGS testing, and provides a minimally invasive option for disease diagnosis and the early detection of key molecular biomarkers essential for the clinical management of women with HGSOC.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70102","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:31:56Z","timestamp":1777350716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring alternatives to tumor tissue for\n                    <i>BRCA1\/2<\/i>\n                    next\u2010generation sequencing testing in high\u2010grade serous ovarian cancer: A 34\u2010case series of malignant ascites"],"prefix":"10.1002","volume":"134","author":[{"given":"Chiara","family":"Pighi","sequence":"first","affiliation":[{"name":"Department of Pathology IRCCS Sacro Cuore Don Calabria Hospital Negrar di Valpolicella  Verona Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulio","family":"Settanni","sequence":"additional","affiliation":[{"name":"Department of Pathology IRCCS Sacro Cuore Don Calabria Hospital Negrar di Valpolicella  Verona 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