{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T19:22:05Z","timestamp":1784402525233,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gene fusion testing, essential for the diagnosis and classification of many salivary gland and soft tissue neoplasms, is typically performed on formalin\u2010fixed paraffin\u2010embedded material, which may be limited or unavailable in cytology specimens. Although cytology smears have been validated for single\u2010gene and DNA\u2010based assays, evidence supporting routine diagnostic use of RNA\u2010based next\u2010generation sequencing fusion testing on smears remains limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The authors evaluated the clinical utility of a clinically validated anchored multiplex polymerase chain reaction (adenosine monophosphate)\u2013based RNA fusion assay performed on digitized, scraped, and sacrificed cytology smears. Alcohol\u2010fixed Papanicolaou\u2010stained and air\u2010dried modified Giemsa\u2010stained smears meeting predefined cellularity thresholds were digitally archived, manually scraped, and processed for RNA extraction. Fusion testing targeted solid tumor\u2013associated genes, and sequencing was performed on an Illumina platform.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of 8706 fusion assays performed during the study period, 807 (9.3%) were applied to cytology specimens, including 14 cases (1.7%) using sacrificed direct smears. Adequate nucleic acid was obtained in all cases. Gene fusions were detected in 64% (9\/14), including\n                      <jats:italic>MYB::NFIB<\/jats:italic>\n                      ,\n                      <jats:italic>ACTB::PLAG1<\/jats:italic>\n                      ,\n                      <jats:italic>NCALD::PLAG1<\/jats:italic>\n                      ,\n                      <jats:italic>CRTC1::MAML2<\/jats:italic>\n                      ,\n                      <jats:italic>COL1A1::PDGFB<\/jats:italic>\n                      ,\n                      <jats:italic>COL1A2::USP6<\/jats:italic>\n                      , and\n                      <jats:italic>FGFR1::NOL4<\/jats:italic>\n                      . Fusion results enabled definitive diagnosis for adenoid cystic carcinoma, mucoepidermoid carcinoma, pleomorphic adenoma, nodular fasciitis, and dermatofibrosarcoma protuberans.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Adenosine monophosphate\u2010based RNA fusion testing on sacrificed cytology smears is feasible, reliable, and diagnostically impactful, expanding the role of cytology specimens in definitive tumor classification.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70105","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:59:11Z","timestamp":1778615951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic utility of anchored multiplex polymerase chain reaction\u2010based fusion assay on sacrificed cytology slides"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3512-8635","authenticated-orcid":false,"given":"Charlotte I.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8029-4787","authenticated-orcid":false,"given":"M. Lisa","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4836-444X","authenticated-orcid":false,"given":"Emilio","family":"Madrigal","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9043-7171","authenticated-orcid":false,"given":"William C.","family":"Faquin","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Chebib","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21214"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21762"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2016.06.002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.25259\/Cytojournal_55_2020"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath\u20102012\u2010201194"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2017.12.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2020\u20100119\u2010CP"},{"key":"e_1_2_7_9_1","unstructured":"MakarenkoVV KimAS.Doing a lot with a little: molecular testing on cytology specimens.Accessed October 25 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