{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:08:45Z","timestamp":1784887725098,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Testing patients with non\u2013small cell lung cancer for actionable variants is essential for guiding treatment decisions in accordance with established cancer care guidelines, though limited quantity and quality of tumor tissue often leaves insufficient material for comprehensive testing. Cytopathology specimens obtained through minimally invasive techniques are a potential source of diagnostic material for genomic profiling, though typically challenging to analyze.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 85 DNA or total nucleic acid non\u2013small cell lung cancer samples derived from 45 fine\u2010needle aspirate rinse or pleural fluid samples from the Hospital of the University of Pennsylvania archive were tested using the Aspyre Clinical Test for Lung (Tissue) in Biofidelity\u2019s CAP\/CLIA laboratory. All samples were previously characterized by the Oncomine Precision Assay Genexus assay (the orthogonal reference method).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Eighty\u2010four of 85 passed Aspyre Lung quality control, one failed. Twenty\u2010six samples were positive for variants in the Aspyre Lung panel: 17 for single nucleotide variants (\n                      <jats:italic>KRAS<\/jats:italic>\n                      ,\n                      <jats:italic>EGFR<\/jats:italic>\n                      ), three for\n                      <jats:italic>EGFR<\/jats:italic>\n                      insertions\/deletions, two for\n                      <jats:italic>MET<\/jats:italic>\n                      exon 14 skipping, and five for gene fusions. Eighty\u2010two of 85 samples were run at standard input levels; three of 85 were run at low input but passed Aspyre Lung controls and include one\n                      <jats:italic>EGFR<\/jats:italic>\n                      exon 20 insertion variant\u2010positive. All results were concordant between methods. Positive Percent Agreement and Negative Percent Agreement were 100%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Aspyre Clinical Test for Lung performs effectively on samples derived from fine needle aspirate rinses and pleural fluid. Using these cytology\u2010based specimens for biomarker testing enables pathologists to perform simplified genomic profiling while preserving valuable tissue specimens, potentially reducing the need for additional invasive procedures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70121","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T07:05:22Z","timestamp":1780902322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Successful simplified genomic profiling of cytology specimens using Aspyre Clinical Test for Lung (Tissue)"],"prefix":"10.1002","volume":"134","author":[{"given":"Caren","family":"Gentile","sequence":"first","affiliation":[{"name":"Hospital of the University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Herlihy","sequence":"additional","affiliation":[{"name":"Hospital of the University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elyse","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Biofidelity Inc  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan Thomas","family":"Evans","sequence":"additional","affiliation":[{"name":"Biofidelity Inc  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Shull","family":"Green","sequence":"additional","affiliation":[{"name":"Biofidelity Inc  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candace","family":"King","sequence":"additional","affiliation":[{"name":"Biofidelity Inc  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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