{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:09:08Z","timestamp":1784887748667,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Malignant effusions in breast cancer are a minimally invasive source for molecular profiling. Effusion\u2010derived cytology specimens, cell blocks (CB) and post\u2010ThinPrep PreservCyt (PTPC) supernatant, are reliable sources for molecular testing but differ in DNA concentration and turnaround time (TAT). This study evaluates mutational profiles, cytology characteristics, and clinical correlates to assess the utility of effusion\u2010based molecular testing in breast cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The authors screened 2400 malignant effusion cases, identifying 22 cases of breast adenocarcinoma effusions that underwent next\u2010generation sequencing (NGS). Both CB and PTPC supernatant were evaluated for tumor cellularity, fluid volume, DNA concentration, TAT, and mutation detection. Associations with clinicopathological features and outcomes were explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NGS was successful in all 22 cases (mean volume, 426.5 mL; mean tumor cellularity, 50%). Successful sequencing was achieved in low volume samples with high tumor cellularity. Higher tumor cellularity (&gt;50%) was associated with bloody effusions (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.016). PTPC supernatant yielded 5\u2010fold higher DNA (mean 177.4 ng\/\u00b5L vs. 33.8 ng\/\u00b5L) and shorter TAT by 9 days (15 vs. 24 days).\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations were more common in estrogen receptor (ER)\u2010negative and triple\u2010negative breast effusions (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.025). Mutations in\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      ,\n                      <jats:italic>TP53<\/jats:italic>\n                      ,\n                      <jats:italic>CCND1<\/jats:italic>\n                      , and\n                      <jats:italic>AKT<\/jats:italic>\n                      were reliably detected. Time from diagnosis to effusion development was significantly shorter in ER negative disease (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.001)\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Effusion cytology using PTPC supernatant, support successful NGS even in low\u2010volume samples. ER\u2010negative and triple\u2010negative effusions demonstrate more aggressive molecular features including enrichment of\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations and earlier effusion development. Effusion\u2010based molecular testing yields clinically relevant genomic information in advanced breast cancer.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70125","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:14:28Z","timestamp":1782803668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular profiling of breast cancer pleural effusions using cytology specimens: Impact of sample source and cytological features on next\u2010generation sequencing performance"],"prefix":"10.1002","volume":"134","author":[{"given":"Harpreet","family":"Virk","sequence":"first","affiliation":[{"name":"Department of Pathology University Hospitals Cleveland Medical Center, Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Michael","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospitals Cleveland Medical Center, Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keri Ann","family":"Pfeil","sequence":"additional","affiliation":[{"name":"Clinical Research Biostatistician, UH Center for Clinical Research  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navid","family":"Sadri","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospitals Cleveland Medical Center, Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Bomeisl","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospitals Cleveland Medical Center, Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5123-0602","authenticated-orcid":false,"given":"Aparna","family":"Harbhajanka","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospitals Cleveland Medical Center, Case Western Reserve University  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.17322"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24410"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058\u2010020\u201001345\u2010z"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2018\u20100218\u2010oa"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010018\u20100006\u2010x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2018.05.024"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2019\u20100904\u2010sa"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2018\u20100902\u2010sa"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22126"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath\u20102018\u2010205396"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1104246"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.10.007"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.21037\/tbcr\u201025\u201011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2018.04.125"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23549"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21897"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24548"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016\u20100108\u2010ra"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12265"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2016.04.004"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.25259\/cytojournal_18_2021"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003134820972327"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.9\u20106\u2010606"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549\u2010020\u201005746\u20108"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.15548"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100615\u2010z"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.39"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096502"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058\u2010020\u201001284\u20109"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2020.11.011"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549\u2010022\u201006637\u2010w"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3113"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010025\u201013486\u20105"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.2147\/tcrm.s251668"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20495"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.ccr\u201013\u20102943"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010024\u201001863\u20109"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698\u2010022\u201000303\u20106"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2024.07.001"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27507"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2012.07.023"}],"container-title":["Cancer Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.70125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cncy.70125","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cncy.70125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:54:08Z","timestamp":1784886848000},"score":1,"resource":{"primary":{"URL":"https:\/\/acsjournals.onlinelibrary.wiley.com\/doi\/10.1002\/cncy.70125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/cncy.70125"],"URL":"https:\/\/doi.org\/10.1002\/cncy.70125","archive":["Portico"],"relation":{},"ISSN":["1934-662X","1934-6638"],"issn-type":[{"value":"1934-662X","type":"print"},{"value":"1934-6638","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,29]]},"assertion":[{"value":"2026-02-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70125"}}