{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:05:13Z","timestamp":1791378313978,"version":"4.3.2"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Urine cytology is recommended for noninvasive detection of urothelial carcinoma (UC) but shows limited sensitivity, particularly in low\u2010grade disease. Keratin 17 (K17) has emerged as a promising immunocytochemical marker. This study evaluated the diagnostic performance and interobserver agreement of K17 in a routine setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective cross\u2010sectional study included 184 urine samples from patients undergoing diagnostic evaluation for suspected UC. K17 immunocytochemistry was performed with a fully automated platform and independently evaluated by two blinded raters. Cytology was classified according to The Paris System for Reporting Urinary Cytology. UC status was determined by histology when available or by follow\u2010up and multidisciplinary assessment. Diagnostic accuracy was assessed by receiver operating characteristic (ROC) analysis; interobserver reliability was assessed with the Cohen \u03ba and intraclass correlation coefficient (ICC). Multivariable logistic regression evaluated combined diagnostic approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      K17 expression was significantly higher in UC than in non\u2010UC samples (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a0.001). Across raters, area under the ROC curve (AUC) values ranged from 0.68 to 0.71. Sensitivity was 52.9%\u201365.9%, with a specificity of 73.7%\u201382.8%. Sensitivity ranged from 45.5% to 54.5% for low\u2010grade tumors and from 57.7% to 73.1% for high\u2010grade tumors. In stage\u2010based analyses, sensitivity was 49.2%\u201361.5% for non\u2013muscle\u2010invasive UC and 65%\u201380% for muscle\u2010invasive UC. Combining K17 with cytology numerically improved diagnostic discrimination (AUC value, 0.76; 95% CI, 0.69\u20130.83). Interobserver agreement was moderate to substantial (\u03ba\u00a0=\u00a00.684; ICC,\u00a00.653). Limitations included single\u2010center design, partial histological verification (81%), and post hoc optimized cutoffs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>K17 immunocytochemistry is a reproducible adjunct to urine cytology, with moderate diagnostic performance. It may warrant further evaluation as an adjunctive marker, particularly in low\u2010grade and cytologically equivocal cases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70130","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:02:33Z","timestamp":1783584153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Keratin 17 immunocytochemistry in urine cytology for urothelial carcinoma detection: Diagnostic performance and interobserver agreement in a prospective real\u2010world cohort"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9423-9869","authenticated-orcid":false,"given":"Gregor","family":"Barth","sequence":"first","affiliation":[{"name":"Department of Hematology, Oncology and Palliative Care University Hospital Brandenburg Brandenburg Medical School Theodor Fontane  Brandenburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5918-8472","authenticated-orcid":false,"given":"Stefan","family":"Koch","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences Brandenburg  Brandenburg Germany"},{"name":"Institute of Pathology University Hospital Brandenburg Brandenburg Medical School Theodor Fontane  Brandenburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0787-2763","authenticated-orcid":false,"given":"Thorsten H.","family":"Ecke","sequence":"additional","affiliation":[{"name":"Department of Urology Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin corporate member of Freie Universit\u00e4t Berlin Humboldt\u2013Universit\u00e4t zu Berlin and Berlin Institute of Health  Berlin Germany"},{"name":"Department of Urology Helios Hospital  Bad Saarow Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_8_3_1","volume-title":"S3\u2010Leitlinie Fr\u00fcherkennung, Diagnose, Therapie und Nachsorge des Harnblasenkarzinoms. 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