{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:04:38Z","timestamp":1789992278649,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acsjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Cytopathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The Lung Cancer Compact Panel (cPANEL) is a recently approved highly sensitive multiplex gene panel in Japan that supports both DNA\u2010 and RNA\u2010based next\u2010generation sequencing. Although cytological specimens are acceptable for cPANEL, unfixed cell pellets or dedicated preservation tubes are typically recommended. However, evidence remains limited regarding whether residual liquid\u2010based cytology (LBC) cell suspensions prepared for routine cytological diagnosis can be used directly for cPANEL testing without dedicated molecular preservation or additional preanalytical processing. In this study, we evaluated the feasibility of applying LBC specimens that are widely used in contemporary clinical practice to cPANEL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed DNA and RNA quality in 69 clinical LBC specimens. Among these, 51 specimens containing non\u2010small cell lung cancer cells with previously determined driver alteration status were subjected to cPANEL testing to evaluate assay concordance with clinical companion diagnostic results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DNA integrity was generally well preserved (DNA Integrity Number [DIN]: 6.2\u00a0\u00b1\u00a01.5). In contrast, RNA integrity showed greater variability (DV200: 16.4\u00a0\u00b1\u00a012.1%). ThinPrep\u2010fixed specimens demonstrated lower DIN and DV200 values compared with CytoRich Red\u2010fixed specimens. Although all samples successfully passed the DNA\u2010based cPANEL assay, six cases (11.8%) failed the RNA\u2010based assay, with RNA yield being a major contributing factor. Among the 46 evaluable specimens, concordance was 95.7% and sensitivity was 92.3%, or 88.9% including RNA module failures as cPANEL\u2010negative.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>With appropriate fixative selection and adequate cellularity, cPANEL using clinical LBC specimens may serve as a practical diagnostic platform. We demonstrated that routine LBC specimens can be directly applied to cPANEL without special preanalytical processing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cncy.70135","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T07:59:07Z","timestamp":1786003147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feasibility of routine clinical liquid\u2010based cytology for lung cancer compact panel testing"],"prefix":"10.1002","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0489-535X","authenticated-orcid":false,"given":"Yoshiki","family":"Shinomiya","sequence":"first","affiliation":[{"name":"Center for Development of Advanced Diagnostics Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako C.","family":"Hatanaka","sequence":"additional","affiliation":[{"name":"Center for Development of Advanced Diagnostics Hokkaido University Hospital  Sapporo Hokkaido Japan"},{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asami","family":"Okumura","sequence":"additional","affiliation":[{"name":"Center for Development of Advanced Diagnostics Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayae","family":"Nange","sequence":"additional","affiliation":[{"name":"Center for Development of Advanced Diagnostics Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamiko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Center for Development of Advanced Diagnostics Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Azuma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Asahikawa Medical Center  Asahikawa Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuji","family":"Marukawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science School of Medical Technology Health Sciences University of Hokkaido  Tobetsu Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamato","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuharu","family":"Abiko","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Sato","sequence":"additional","affiliation":[{"name":"DNA Chip Research Inc.  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