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In this study, we determined whether the expression of tyrosine kinase receptors (TrkA, TrkB) is altered in lumbosacral DRG after SCI through immunofluorescence techniques. Complete transection of the spinal cord (T8\u2013T10) was performed in female Wistar rats (120\u2013150 g), and animals were studied 5\u20136 weeks after SCI. One week before killing, Fast Blue (FB) was injected into the bladder to label bladder afferent cells in the L1, L2, L6, and S1 DRG. After SCI, a significant increase in the number of TrkA\u2010immunoreactive (IR) positive cells was detected in the L6\u2010S1 DRG (L6: 1.9\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.01; S1: 1.7\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.05) and in the L1 DRG (3.0\u2010fold; <jats:italic>P<\/jats:italic> \u2264 0.01) but not in the L4\u2010L5 DRG compared with spinal\u2010intact (control) rats. After SCI, a significant increase in the number of TrkB\u2010IR cells was also detected in the L6\u2010S1 DRG (L6: 2.2\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.01; S1: 1.5\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.05) and in the L1\u2010L2 DRG (L1: 1.5\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.01; L2: 1.3\u2010fold, <jats:italic>P<\/jats:italic> \u2264 0.05) but not in the L4\u2010L5 DRG compared with control rats. After SCI, the percentage of FB\u2010labeled cells expressing TrkA immunoreactivity (\u223c68%) or TrkB immunoreactivity (\u223c65%) in L1 and L6 DRG significantly (<jats:italic>P<\/jats:italic> \u2264 0.01) increased compared with control (20\u201330%) DRG. After SCI, the percentage of TrkA\u2010IR cells expressing phosphorylated (p)\u2010Trk immunoreactivity significantly increased (1.5\u2010 to 2.3\u2010fold increase) in the L1, L6, and S1 DRG. The percentage of TrkB\u2010IR cells expressing p\u2010Trk immunoreactivity after SCI also increased (1.3\u2010fold increase) in the L1 and L6 DRG. These results demonstrate that (1) TrkA and TrkB immunoreactivity is increased in bladder afferent cells after SCI and (2) TrkA and TrkB receptors are phosphorylated in DRG after SCI. Neuroplasticity of lower urinary tract reflexes after SCI may be mediated by both nerve growth factor and brain\u2010derived neurotrophic factor. J. Comp. Neurol. 449:217\u2013230, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10283","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T11:32:01Z","timestamp":1033903921000},"page":"217-230","source":"Crossref","is-referenced-by-count":63,"title":["Up\u2010regulation of tyrosine kinase (Trka, Trkb) receptor expression and phosphorylation in lumbosacral dorsal root ganglia after chronic spinal cord (T8\u2013T10) injury"],"prefix":"10.1002","volume":"449","author":[{"given":"Liya","family":"Qiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret 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