{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:40:14Z","timestamp":1758123614502},"reference-count":67,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,6,28]],"date-time":"2002-06-28T00:00:00Z","timestamp":1025222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2002,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rubrospinal motoneurons (RSMN) represent a population of androgen receptor\u2010containing central motoneurons in rodents. In this study, the ability of testosterone propionate (TP), alone or in conjunction with a peripheral nerve graft (PNG), to alter the molecular program of injured RSMN was accomplished using \u03b2II\u2010tubulin cDNA probes and quantitative in situ hybridization (ISH). Initial fluoro\u2010gold labeling experiments following a T1 hemisection established that, as in the rat, the hamster rubrospinal system is essentially crossed and that injured RSMN concentrate in the ventrolateral region of the red nucleus. In the second experimental series, adult gonadectomized male hamsters were subjected to a right T1 hemisection, with half of the operated animals immediately subcutaneously implanted with 1 10 mm TP Silastic capsule and the other half sham implanted. In a third experimental series, animals were subjected to T1 hemisection, followed by transplantation of a predegenerated autologous segment of peripheral nerve. Half of the animals in each group received TP implants at the time of spinal cord injury and PNG. Postoperative times were 2, 7, and 14 days (dpo). Quantitative ISH was performed using a \u03b2II\u2010tubulin\u2010specific <jats:sup>33<\/jats:sup>P\u2010labeled cDNA probe, emulsion autoradiography, and computerized image analysis for grain counting. Injury alone resulted in a short\u2010lived increase in \u03b2II\u2010tubulin mRNA expression in the RSMN at 2 dpo, with a significant decline to well below control values at 7 and 14 dpo. TP treatment or PNG alone attenuated, but did not prevent, the down\u2010regulation of \u03b2II\u2010tubulin mRNA. In contrast, the combination of TP with a PNG sustained the injury\u2010induced increase in \u03b2II\u2010tubulin mRNA levels throughout the postoperative period of 2, 7, and 14 dpo. The synergistic effects of the two treatment strategies confirm the importance of targeting multiple aspects of the injury response for therapeutic intervention. J. Comp. Neurol. 449:364\u2013373, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10304","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:33:49Z","timestamp":1030318429000},"page":"364-373","source":"Crossref","is-referenced-by-count":12,"title":["Combination of gonadal steroid treatment and peripheral nerve grafting results in a peripheral motoneuron\u2010like pattern of BII\u2010tubulin mRNA expression in axotomized hamster rubrospinal motoneurons"],"prefix":"10.1002","volume":"449","author":[{"given":"Paul D.","family":"Storer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Houle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Oblinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn J.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,6,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)64018-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.07.030184.002213"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(20000115)29:2<175::AID-GLIA11>3.0.CO;2-F"},{"key":"e_1_2_6_5_1","first-page":"79","volume-title":"Neural regeneration and transplantation","author":"Barron KD","year":"1989"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/296150a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02780547"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.4.7.1313"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6705"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050943"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991129)414:4<495::AID-CNE6>3.0.CO;2-S"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1139\/o95-073"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-12-04623.1997"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00025-3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.8.1592204"},{"key":"e_1_2_6_16_1","first-page":"192","article-title":"Distinct roles of neurofilament and tubulin gene expression in axonal growth","volume":"138","author":"Hoffman PN","year":"1988","journal-title":"Ciba Found Symp"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-03-00893.1989"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.12.4530"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(91)90139-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2000.053"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1998.6850"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1242\/jcs.103.3.643","article-title":"Differential regulation of \u03b2III and other tubulin genes during peripheral and central neuron development","volume":"103","author":"Jiang YQ","year":"1992","journal-title":"J Cell Sci"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01001350"},{"key":"e_1_2_6_24_1","first-page":"227","article-title":"Gonadal steroids and neuronal regeneration. 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