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In contrast to the subsequent massive secondary degeneration of the granule cells and the inferior olivary neurons, only mild degeneration occurs in the deep cerebellar nuclei (DCN). However, it is not known to what extent the different populations of DCN neurons\u2014glutamatergic principal projection neurons, \u03b3\u2010aminobutyric acid (GABA)\u2010ergic inferior olivary projection neurons, and glycinergic neurons\u2014are affected in their neurotransmitter composition. To answer this question we studied the neurotransmitter contents (glutamate, GABA, and glycine) of DCN neurons and the size of synaptic boutons immunohistochemically on serial semithin sections in both <jats:italic>Lurcher<\/jats:italic> and wild\u2010type mice. Applying the physical dissector counting method, our results confirmed the mild degeneration (a reduction by 20%) of large glutamatergic neurons and a more pronounced degeneration of GABAergic (by 42%) and glycinergic neurons (by 45%). On the other hand, an analysis of neurons colabeled for both GABA and glycine, revealed that this specific colabeling increased in the <jats:italic>Lurcher<\/jats:italic> mutant (by 40%). In addition, both the GABA\u2010immunolabeled (IL) (by 56%) and the glycine\u2010IL (by 45%) synaptic boutons showed an increase in diameter in the mutant. The density of these boutons showed a decrease of 30% each. In summary, the increase in the number of neurons colabeled for GABA and glycine, together with the increase in the size of the inhibitory synaptic boutons, could help in providing the minimum inhibition needed to maintain a residual \u201ccerebellar\u201d functionality in the <jats:italic>Lurcher<\/jats:italic> DCN. J. Comp. Neurol. 452:311\u2013323, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10365","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T04:46:32Z","timestamp":1034138792000},"page":"311-323","source":"Crossref","is-referenced-by-count":63,"title":["Quantitative organization of neurotransmitters in the deep cerebellar nuclei of the Lurcher 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