{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:58:10Z","timestamp":1791079090012,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,12,30]],"date-time":"2002-12-30T00:00:00Z","timestamp":1041206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Biotechnology Centre for Cellular Communication"},{"DOI":"10.13039\/100008392","name":"Danish Medical Research Council","doi-asserted-by":"crossref","award":["j.nr.9701798"],"award-info":[{"award-number":["j.nr.9701798"]}],"id":[{"id":"10.13039\/100008392","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Vaern om Synet"},{"name":"Velux Foundation"},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004954","name":"Augustinus Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004954","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Danish Medical Association Research Fund (Laegeforeningens forskningsfond, H\u00f8jmosegaardslegatet)"},{"name":"R\u00e9gion Centre, France"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2003,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the sheep, the pineal hormone melatonin displays nocturnal levels 20 times as high in the cerebrospinal fluid of the third ventricle as in the jugular blood. Moreover, in the pineal recess, the evagination of the third ventricle into the pineal stalk, the levels of melatonin in the cerebrospinal fluid are even higher than in the ventral part of the third ventricle. This finding suggests melatonin to be secreted directly from the pineal gland to the ventricular lumen of the pineal recess of this species. We have, therefore, studied the interface between the sheep pineal gland and the cerebrospinal fluid by light\u2010, scanning\u2010, and electron microscopy of the pineal recess, as well as the permeability of the interface by tracer injections into the third ventricle. First, we show that the classic ependymal lining of the third ventricle disappears in the superior part of the recess. In this area, bulging pinealocytes, displaying immunoreactivity for serotonin, directly appose the cerebrospinal fluid. This pineal\u2013cerebrospinal fluid interface of the sheep is large compared with other species, especially rodent species. Intraventricular injections of horseradish peroxidase and fluorescein isothiocyanate showed that both these tracers could permeate from the pineal recess into the sheep pineal parenchyma. This permeation was due to the presence of gap and intermediate junctions connecting the pinealocytes apposing the ventricular lumen. Thus, our results show that endocrine cells in this specialized area of the ventricular system are in direct contact with the cerebrospinal fluid. This finding supports the physiological concept of a direct secretion of melatonin into the cerebrospinal fluid of the sheep pineal recess. J. Comp. Neurol. 456:39\u201347, 2003. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10477","type":"journal-article","created":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T02:21:47Z","timestamp":1041387707000},"page":"39-47","source":"Crossref","is-referenced-by-count":32,"title":["Cellular lining of the sheep pineal recess studied by light\u2010, transmission\u2010, and scanning electron microscopy: Morphologic indications for a direct secretion of melatonin from the pineal gland to the cerebrospinal fluid"],"prefix":"10.1002","volume":"456","author":[{"given":"H\u00e9l\u00e8ne","family":"Tricoire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Malpaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten","family":"M\u00f8ller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051228"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(94)90153-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0024-3205(94)00728-4","article-title":"Infusion of a pineal gland\u2010derived antigonadotropic decapeptide into the lateral ventricle depresses prolactin levels in male rats","volume":"55","author":"Benson 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