{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:07:37Z","timestamp":1791403657318,"version":"4.3.3"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,1,15]],"date-time":"2003-01-15T00:00:00Z","timestamp":1042588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2003,2,17]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuritic plaques are one of the stereotypical hallmarks of Alzheimer's disease (AD) pathology. These structures are composed of extracellular accumulations of fibrillar forms of the amyloid\u2010\u03b2 peptide (A\u03b2), a variety of other plaque\u2010associated proteins, activated glial cells, and degenerating nerve processes. To study the neuritic toxicity of different structural forms of A\u03b2 in the context of regional connectivity and the entire cell, we crossed PDAPP transgenic (Tg) mice, a model with AD\u2010like pathology, to Tg mice that stably express yellow fluorescent protein (YFP) in a subset of neurons in the brain. In PDAPP; YFP double Tg mice, markedly enlarged YFP\u2010labeled axonal and dendritic varicosities were associated with fibrillar A\u03b2 deposits. These varicosities were absent in areas where there were nonfibrillar A\u03b2 deposits. Interestingly, YFP\u2010labeled varicosities revealed changes that corresponded with changes seen with electron microscopy and the de Olmos silver staining technique. Other silver staining methods and immunohistochemical localization of phosphorylated neurofilaments or phosphorylated tau were unable to detect the majority of these dystrophic neurites. Some but not all synaptic vesicle markers accumulated abnormally in YFP\u2010labeled varicosities associated with neuritic plaques. In addition to the characterization of the effects of A\u03b2 on axonal and dendritic structure, YFP\u2010labeled neurons in Tg mice should prove to be a valuable tool to interpret the localization patterns of other markers and for future studies examining the dynamics of axons and dendrites in a variety of disease conditions in living tissue both in vitro and in vivo. J. Comp. Neurol. 456:375\u2013383, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10536","type":"journal-article","created":{"date-parts":[[2003,1,17]],"date-time":"2003-01-17T16:46:22Z","timestamp":1042821982000},"page":"375-383","source":"Crossref","is-referenced-by-count":48,"title":["PDAPP; YFP double transgenic mice: A tool to study amyloid\u2010\u03b2 associated changes in axonal, dendritic, and synaptic structures"],"prefix":"10.1002","volume":"456","author":[{"given":"Robert P.","family":"Brendza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Simmons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel 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