{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:41:09Z","timestamp":1761057669870},"reference-count":69,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T00:00:00Z","timestamp":1049328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2003,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neurons in rat superficial dorsal horn that express neurokinin receptor 1 (NK1), a receptor for substance P, play a critical role in the development of hyperalgesia. Thermal hyperalgesia is dramatically reduced after ablation of these neurons, but, paradoxically, not in mice that lack the NK1 receptor (Mantyh et al. [1997] Science 278:275\u2013279). Because primary afferents that express vanilloid receptor 1 (VR1), a receptor for noxious heat, are essential for thermal nociception and hyperalgesia, we reasoned that VR1\u2010positive fibers may terminate onto NK1\u2010expressing dorsal horn neurons. We therefore combined immunofluorescent staining for VR1 and NK1 to show that NK1\u2010positive neurons in lamina I are contacted by VR1\u2010positive fibers. That these contacts represent synapses was verified by staining for the presynaptic marker synaptophysin and by electron microscopy. By combining retrograde tracing with immunocytochemistry, we also found that most NK1\u2010positive cells contacted by VR1\u2010positive fibers project to the lateral parabrachial nucleus. Because quantitative evaluation suggests a preferential targeting of NK1\u2010positive lamina I neurons by fibers containing VR1, these results demonstrate a significant monosynaptic innervation of spinoparabrachial neurons by VR1\u2010positive afferents. J. Comp. Neurol. 460:255\u2013265, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.10647","type":"journal-article","created":{"date-parts":[[2003,4,7]],"date-time":"2003-04-07T15:02:36Z","timestamp":1049727756000},"page":"255-265","source":"Crossref","is-referenced-by-count":31,"title":["VR1\u2010positive primary afferents contact NK1\u2010positive spinoparabrachial neurons"],"prefix":"10.1002","volume":"460","author":[{"given":"Se Jin","family":"Hwang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Burette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juli 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