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In this study, we used two different techniques\u2014nicotinamide adenine dinucleotide phosphate\u2010diaphorase (NADPH\u2010d) histochemistry and NOS immunocytochemistry\u2014to demonstrate that NOS is present in a pair of identifiable cells in the central nervous system of the nudibranch <jats:italic>Melibe leonina<\/jats:italic>. In the <jats:italic>Melibe<\/jats:italic> brain, NADPH\u2010d histochemistry revealed only a single pair of bilaterally symmetrical cells in the cerebropleural ganglia. NOS activity also was found in the neuropil of the cerebral, pedal, and buccal ganglia; in the tentacles of the oral hood; in the sensory end of the rhinophores; and in the epithelial tissue of the mouth, preputium, and glans penis. Immunocytochemistry using NOS antisera corroborated the results of the NADPH\u2010d histochemistry by staining the same two cells in the cerebropleural ganglia. Each of these identifiable nitrergic neurons projects into the ipsilateral pedal ganglion. Because the pedal ganglia play a critical role in the control of locomotion, our results provide morphological evidence suggesting that NO may influence swimming or crawling in <jats:italic>Melibe leonina<\/jats:italic>. J. Comp. Neurol. 437:70\u201378, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.1270","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:49:13Z","timestamp":1030312153000},"page":"70-78","source":"Crossref","is-referenced-by-count":21,"title":["Identifiable nitrergic neurons in the central nervous system of the nudibranch <i>Melibe leonina<\/i> localized with NADPH\u2010diaphorase histochemistry and nitric oxide synthase immunoreactivity"],"prefix":"10.1002","volume":"437","author":[{"given":"James M.","family":"Newcomb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Winsor H.","family":"Watson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,7,20]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"269","article-title":"Notes on Melibe leonina (Gould) [plates 49 and 50]","volume":"2","author":"Agersborg HPvWK","year":"1919","journal-title":"Publ Puget Sound Biol Sta"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/279809"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00235308"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90104-L"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90374-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00300230"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.17.7797"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199303000-00013"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-11-07653.1995"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.4.1444"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.003343"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/336385a0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/369061a0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1983.49.4.1017"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1983.49.4.1036"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1980.44.1.151"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2811"},{"key":"e_1_2_6_19_1","first-page":"151","article-title":"The feeding mechanism and behaviour of the opisthobranch Melibe leonina","volume":"22","author":"Hurst A","year":"1968","journal-title":"Symp Zool Soc London"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.2.891"},{"key":"e_1_2_6_21_1","first-page":"524","article-title":"Nitric oxide as a putative transmitter in Aplysia: neural circuits and membrane effects","volume":"44","author":"Jacklet JW","year":"1994","journal-title":"Netherlands J Zool"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.5.1900"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.81.2.654"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199804)35:1<65::AID-NEU6>3.0.CO;2-9"},{"key":"e_1_2_6_25_1","unstructured":"LawrenceKA.1997.Neuroethology of the swimming behavior in the pacific nudibranch Melibe leonina[Ph.D. dissertation]. 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