{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:55:43Z","timestamp":1757591743550},"reference-count":94,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,7,23]],"date-time":"2001-07-23T00:00:00Z","timestamp":995846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2001,8,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The distribution of atrial natriuretic factor (ANF)\u2010like immunoreactivity in the central nervous system of the cave salamander <jats:italic>Hydromantes genei<\/jats:italic> (Amphibia, Plethodontidae) was investigated by using antisera raised against rat and human ANF(1\u201328). Concurrently, the location of ANF\u2010binding sites was determined by autoradiography, using radioiodinated human ANF(1\u201328) as a tracer. In several regions of the brain, including the olfactory bulb, the preoptic area, the ventral thalamus, the tectum of the mesencephalon, and the choroid plexuses inside the ventricles, a good correlation was observed between the distribution of ANF\u2010immunoreactive elements and the location of ANF\u2010binding sites. Mismatching was found in the habenular nucleus, the commissura habenularis, the fasciculum retroflexus, and the interpeduncular nucleus, which contained high levels of binding sites but were devoid of ANF\u2010immunoreactive structures. In contrast, a few other regions, such as the pineal gland and the subcommissural organ, showed a high concentration of ANF\u2010like immunoreactivity but did not contain ANF\u2010binding sites. This study provides the first localization of ANF\u2010like immunoreactivity and ANF\u2010binding sites in the brain of an urodele amphibian. The results show that the ANF peptidergic system in the cave salamander has an organization more simple than the organizations described for the brain of frog or other vertebrates. This feature is probably related to the expression of highly pedomorphic characters in plethodontids. The anatomical distribution of ANF\u2010immunoreactive elements and ANF\u2010binding sites suggests that ANF\u2010related peptides may act as hypophysiotropic hormones as well as neurotransmitters and\/or neuromodulators in the salamander brain. J. Comp. Neurol. 437:240\u2013258, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.1281","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:50:36Z","timestamp":1030312236000},"page":"240-258","source":"Crossref","is-referenced-by-count":3,"title":["Immunohistochemical localization of atrial natriuretic factor and autoradiographic distribution of atrial natriuretic factor\u2010binding sites in the brain of the cave salamander <i>Hydromantes genei<\/i> (Amphibia, 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