{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T01:19:42Z","timestamp":1773623982921,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,7,31]],"date-time":"2001-07-31T00:00:00Z","timestamp":996537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2001,8,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Different levels of the cutaneous vasculature are innervated selectively by subpopulations of sympathetic neurons distinguished by the presence or absence of immunoreactivity (\u2010IR) for neuropeptide Y (NPY). This study used multiple\u2010labelling immunohistochemistry to examine the appearance of NPY\u2010IR in neurons innervating cutaneous vessels in the ear pinna of embryonic, fetal, and neonatal guinea pigs. NPY\u2010immunoreactive axons were detected in the ear bud at embryonic day 25. However, these axons lacked IR for tyrosine hydroxylase (TH) and often ran in bundles with substance P (SP)\u2010immunoreactive axons close to the epidermis. Many neuronal somata in the cervical dorsal root ganglia (DRG) at late embryonic stages contained NPY\u2010IR with or without SP\u2010IR, but no NPY\u2010IR was detected in DRG or subepidermal axons by late fetal stages. IR for calcitonin gene\u2010related peptide increased in DRG neurons from midfetal to late fetal stages, after the decrease in NPY\u2010IR. Populations of TH\u2010IR neurons with or without NPY\u2010IR were present in the superior cervical ganglion (SCG) from midembryonic stages. TH\u2010immunoreactive axons were not detected in the ear pinna until midfetal stages, when axons with TH\u2010IR and NPY\u2010IR innervated proximal arteries and TH\u2010immunoreactive axons without NPY\u2010IR innervated distal vessels. Vasoactive intestinal peptide\u2010IR was detected transiently in most fetal SCG neurons with TH\u2010IR and NPY\u2010IR but was not detected in cutaneous axons. These results demonstrate that selective expression of NPY by subpopulations of sympathetic neurons occurs prior to innervation of their targets. This suggests that target contact is not required to establish appropriate patterns of expression of peptide neurotransmitters by cutaneous sympathetic neurons. J. Comp. Neurol. 437:321\u2013334, 2001.\u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.1286","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:50:36Z","timestamp":1030312236000},"page":"321-334","source":"Crossref","is-referenced-by-count":11,"title":["Neuropeptide Y immunoreactivity in cutaneous sympathetic and sensory neurons during development of the guinea pig"],"prefix":"10.1002","volume":"437","author":[{"given":"Judy L.","family":"Morris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian 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