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The 3\u2032UTR of <jats:italic>t<\/jats:italic>\u2010<jats:italic>PA<\/jats:italic> mRNA is known to be involved in the reversible deadenylation and translational repression of transcripts in mouse oocytes. <jats:italic>hCMV<\/jats:italic>\/\u03b2\u2010<jats:italic>actin<\/jats:italic>\u2010<jats:italic>eGFP<\/jats:italic>\u2010<jats:italic>3<\/jats:italic>\u2032<jats:italic>UTR t<\/jats:italic>\u2010<jats:italic>PA<\/jats:italic> transgenic mice express <jats:italic>eGFP<\/jats:italic> mRNA in all brain structures analyzed but lack eGFP fluorescence, with the exception of blood vessels, choroid plexus, and Purkinje cells. Taking advantage of these features, we tested whether certain pathological conditions, in particular injuries of the nervous system, might trigger eGFP fluorescence in traumatized cells or neurons. From this perspective, we analyzed <jats:italic>eGFP<\/jats:italic> mRNA expression and eGFP fluorescence in experimental models of nervous system lesions, such as motoneuron axotomy and cerebral stroke induced by middle cerebral artery occlusion. We found an increase in eGFP fluorescence in specific brain areas in cells suffering or reacting to these injuries. This increased fluorescence is correlated with an increased transcription of <jats:italic>eGFP<\/jats:italic> in lesioned cells, presumably enhanced by a release of the translational silencing mediated by the 3\u2032UTR region of the t\u2010PA mRNA. This transgenic mouse model may prove useful to study the development of neurodegenerative lesions. J. Comp. Neurol. 474:108\u2013122, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.20122","type":"journal-article","created":{"date-parts":[[2004,5,17]],"date-time":"2004-05-17T20:01:13Z","timestamp":1084824073000},"page":"108-122","source":"Crossref","is-referenced-by-count":6,"title":["Induction of enhanced green fluorescent protein expression in response to lesions in the nervous 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