{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:29:17Z","timestamp":1790634557207,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T00:00:00Z","timestamp":1085529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2004,6,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Developing olfactory sensory neurons are guided to the glomeruli of the olfactory bulb by an increasingly stringent process that is influenced by expression of odorant receptors, cell adhesion molecules (CAMs), and other kinds of signaling cascades. A fundamental feature of the projection is the connecting of broad zones in the epithelium to broad zones in the bulb, also termed <jats:italic>rhinotopy<\/jats:italic>. One molecule that parallels and may aid neurons in establishing rhinotopy is the mammalian homologue of fasciclin II (OCAM\/mamFas II; also known as <jats:italic>RNCAM<\/jats:italic> and <jats:italic>NCAM\u20102<\/jats:italic>), an immunoglobulin superfamily CAM that is differentially expressed in the developing and mature olfactory epithelium (OE): Axons elaborated by ventral and lateral epithelium express the protein at high levels, whereas dorsomedial axons express little or no OCAM\/mamFas II. Our investigation has demonstrated that OCAM\/mamFas II is detectable early in the development of the rat OE. mRNA is evident on RT\u2010PCR and in situ hybridization by E12.5, and protein is apparent by immunohistochemistry by E13.5. By using a tissue culture system that separates ventral septal epithelium (OCAM\/mamFas II\u2010positive) from dorsal (OCAM\/mamFas II\u2010negative), we find that explants maintain protein expression levels in vitro that are characteristic of the phenotype at the original location in vivo. At least some neurons are born in culture, suggesting that any cues that direct differential expression are also maintained in vitro. Finally, high OCAM\/mamFas II expression correlates with increased growth and fasciculation of olfactory axons in vitro. These data and the similarity between OCAM\/mamFas II, on the one hand, and fasciclin II and NCAM, on the other, suggest that OCAM\/mamFas II might play a role in growth and fasciculation of primary olfactory axons during development of the projection. J. Comp. Neurol. 474:438\u2013452, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.20133","type":"journal-article","created":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T17:02:46Z","timestamp":1085590966000},"page":"438-452","source":"Crossref","is-referenced-by-count":26,"title":["Differential expression of the mammalian homologue of fasciclin II during olfactory development in vivo and in vitro"],"prefix":"10.1002","volume":"474","author":[{"given":"John A.","family":"Hamlin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengsheng","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James 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