{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:19:26Z","timestamp":1791299966599,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T00:00:00Z","timestamp":1085529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2004,6,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The dorsal raphe nucleus (DR) has a topographic neuroanatomy consistent with the idea that different parts of this nucleus subserve different functions. Here we use dual in situ hybridization to describe the rostral\u2010caudal neurochemical distribution of three major cell groups, serotonin (5\u2010hydroxytryptamine; 5\u2010HT), \u03b3\u2010aminobutyric acid (GABA), and catecholamine, and their relative colocalization with each other and mRNA encoding four different receptor subtypes that have been described to influence DR responses, namely, 5HT\u20101A, \u03b1<jats:sub>1b<\/jats:sub> adrenergic (\u03b1<jats:sub>1b<\/jats:sub> ADR), and corticotropin\u2010releasing factor type 1 (CRF\u2010R1) and 2 (CRF\u2010R2) receptors. Serotonergic and GABAergic neurons were distributed throughout the rostral\u2010caudal extent of the DR, whereas catecholaminergic neurons were generally restricted to the rostral half of the nucleus. These phenotypes essentially represent distinct cell populations, because the neurochemical markers were rarely colocalized. Both 5HT\u20101A and \u03b1<jats:sub>1b<\/jats:sub> ADR mRNA were highly expressed throughout the DR, and the vast majority of serotonergic neurons expressed both receptors. A smaller percentage of GABAergic neurons also expressed 5HT\u20101A or \u03b1<jats:sub>1b<\/jats:sub> ADR mRNA. Very few catecholaminergic cells expressed either 5HT\u20101A or \u03b1<jats:sub>1b<\/jats:sub> ADR mRNA. CRF\u2010R1 mRNA was detected only at very low levels within the DR, and quantitative colocalization studies were not technically feasible. CRF\u2010R2 mRNA was mainly expressed at the middle and caudal levels of the DR. At midlevels, CRF\u2010R2 mRNA was expressed exclusively in serotonin neurons, whereas, at caudal levels, approximately half the CRF\u2010R2 mRNA was expressed in GABAergic neurons. The differential distribution of distinct neurochemical phenotypes lends support to the idea of functional differentiation of the DR. J. Comp. Neurol. 474:364\u2013378, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.20138","type":"journal-article","created":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T17:02:30Z","timestamp":1085590950000},"page":"364-378","source":"Crossref","is-referenced-by-count":182,"title":["Differential expression of 5HT\u20101A, \u03b1<sub>1b<\/sub> adrenergic, CRF\u2010R1, and CRF\u2010R2 receptor mRNA in serotonergic, \u03b3\u2010aminobutyric acidergic, and catecholaminergic cells of the rat dorsal raphe nucleus"],"prefix":"10.1002","volume":"474","author":[{"given":"Heidi E.W.","family":"Day","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin N.","family":"Greenwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayamwong E.","family":"Hammack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda R.","family":"Watkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Fleshner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven F.","family":"Maier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Campeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,5,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(80)90187-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(80)90461-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90646-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)90994-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-03-01142.2000"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.275.5.R1438"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-10-06340.1995"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-0618(98)00036-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000508)420:3<305::AID-CNE3>3.0.CO;2-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300045"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-0618(97)00042-2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991011)413:1<113::AID-CNE8>3.0.CO;2-B"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902490407"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903630203"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(79)91527-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(95)02135-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01208-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(02)00061-X"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-07-02889.2003"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1523\/JNEUROSCI.22-03-01020.2002","article-title":"The role of corticotropin\u2010releasing hormone in the dorsal raphe nucleus in mediating the behavioral consequences of uncontrollable stress","volume":"22","author":"Hammack SE","year":"2002","journal-title":"J Neurosci"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1523\/JNEUROSCI.23-03-01019.2003","article-title":"Corticotropin releasing hormone type 2 receptors in the dorsal raphe nucleus mediate the behavioral consequences of uncontrollable stress","volume":"23","author":"Hammack SE","year":"2003","journal-title":"J Neurosci"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90336-8"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902700402"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1037\/0097-7403.6.1.21"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.1.165"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(99)00012-3"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3223(88)90091-1"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(99)00093-7"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00584-5"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1033"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(82)90054-9"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02468-9"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-20-07728.2000"},{"key":"e_1_2_6_35_1","volume-title":"2002 Abstract Viewer\/Itinerary Planner","author":"Lowry CA","year":"2002"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.107.2.377"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.108.1.121"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.109.3.404"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01313-9"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893-133X(96)00093-0"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90281-5"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1037\/0097-7403.1.4.364"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0024166"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209532200"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960115)364:3<402::AID-CNE2>3.0.CO;2-8"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00268-6"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(84)90100-6"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-03-00970.2003"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1515\/REVNEURO.1997.8.2.117"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00248288"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01557.x"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(93)90128-G"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890010103"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01631-3"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00119-B"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(81)90146-9"},{"key":"e_1_2_6_57_1","first-page":"157","article-title":"The nucleus raphe dorsalis of the rat and its projection upon the caudatoputamen. A combined cytoarchitectonic, immunohistochemical and retrograde transport study","volume":"77","author":"Steinbusch HW","year":"1981","journal-title":"J Physiol"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990510)407:3<367::AID-CNE5>3.0.CO;2-6"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90239-8"},{"key":"e_1_2_6_60_1","volume-title":"Brain maps: structure of the rat brain","author":"Swanson LW","year":"1992"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90077-Z"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001211)428:2<191::AID-CNE1>3.0.CO;2-U"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903510304"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(85)90481-1"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.20138","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.20138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T20:01:12Z","timestamp":1700251272000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.20138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,26]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,6,28]]}},"alternative-id":["10.1002\/cne.20138"],"URL":"https:\/\/doi.org\/10.1002\/cne.20138","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5,26]]}}}