{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:50:31Z","timestamp":1761580231612},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,8,20]],"date-time":"2004-08-20T00:00:00Z","timestamp":1092960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2004,10,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the present immunohistochemical study was to investigate the localization of neurokinin 1 receptor (NK1R) in rat esophagus and examine the relationship between NK1Rs and intrinsic cholinergic, nitrergic, or substance P (SP) neurons. NK1R immunoreactivity (IR) was observed on the nerve cell bodies in the myenteric ganglia throughout the esophagus, but not on striated muscles and smooth muscle cells of the muscularis mucosae. The frequency of occurrence of NK1R neurons was highest in the cervical esophagus and lowest in the lower thoracic esophagus. Considerable immunoreactivity was seen on the nerve cell surfaces and was also present in the cytoplasm of cell somas and in the initial part of the axons, but not in any other nerve fibers or terminals. Dogiel type I\u2010like morphology was observed in some of the NK1R neurons; however, the majority exhibited polymorphic morphology. Double immunolabeling indicated that a majority (77%) of the NK1R neurons were immunoreactive for choline acetyltransferase (ChAT), while a minority (23%) were immunoreactive for nitric oxide synthase (NOS)\u2010IR. Most of the NK1R neurons (92%) were innervated by the SP nerve fibers. Triple immunolabeling indicated that 70% of the NK1R neurons were associated with intrinsic SP nerve fibers (without CGRP\u2010IR), 59% were associated with extrinsic SP nerve fibers (with CGRP\u2010IR), and 35% were associated with both intrinsic and extrinsic SP nerve fibers. These results suggest that SP\/tachykinin released from the SP nerve fibers of intrinsic and\/or extrinsic origin activates the predominantly intrinsic cholinergic neurons via NK1Rs to influence neuronal transmission or motility in rat esophagus. J. Comp. Neurol. 478:11\u201321, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.20169","type":"journal-article","created":{"date-parts":[[2004,8,20]],"date-time":"2004-08-20T19:09:29Z","timestamp":1093028969000},"page":"11-21","source":"Crossref","is-referenced-by-count":11,"title":["Localization of neurokinin 1 receptor (NK1R) immunoreactivity in rat esophagus"],"prefix":"10.1002","volume":"478","author":[{"given":"Hirofumi","family":"Kuramoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Oomori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Murabayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kadowaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Ichiro","family":"Karaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsukazu","family":"Kuwahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902160106"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(98)00459-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.2000.00200.x"},{"key":"e_1_2_6_5_1","first-page":"93","article-title":"Pharmacological properties of mechanical responses of the rat oesophageal muscularis mucosae to vagal and field stimulation","volume":"84","author":"Bieger D","year":"1985","journal-title":"Br J Pharmacol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(97)00083-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(84)90583-1"},{"key":"e_1_2_6_8_1","first-page":"595","volume-title":"Physiology of the gastrointestinal tract","author":"Christensen J","year":"1987"},{"key":"e_1_2_6_9_1","first-page":"903","volume-title":"Physiology of the gastrointestinal tract","author":"Conklin JL","year":"1994"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890060410"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(94)00921-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02407391"},{"key":"e_1_2_6_13_1","first-page":"130","article-title":"Ueber den bau der Ganglien in den Geflechten des Darmes und der Gallenblase des Menschen und der Saugetiere","author":"Dogiel AS","year":"1899","journal-title":"Arch Anat Physiol Leipzig, Anat Abt"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980824)398:2<289::AID-CNE9>3.0.CO;2-X"},{"key":"e_1_2_6_15_1","first-page":"24","article-title":"Differential activation of intracellular effector by two isoforms of human neurokinin\u20101 receptor","volume":"41","author":"Fong TM","year":"1992","journal-title":"Mol Pharmacol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90707-5"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(78)90160-0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0197-0186(91)90180-L"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890050209"},{"key":"e_1_2_6_20_1","first-page":"236","article-title":"Coexistence of three tachykinin receptors coupled to Ca++ signaling pathways in intestinal muscle cells","author":"Hellestr\u00f6m PM","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00455243"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(96)00195-7"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051156"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1113\/jphysiol.2003.044669","article-title":"Role of intrinsic nitrergic neurons on vagally mediated striated muscle contractions in the hamster oesophagus","volume":"551","author":"Izumi N","year":"2003","journal-title":"J Physiol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb05857.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1995.tb09903.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701001"},{"key":"e_1_2_6_28_1","first-page":"78","volume-title":"Neurogastroenterology: from the basics to the clinics","author":"Kuramoto H","year":"2000"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11424-U"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00305778"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s004410051230","article-title":"Motor innervation by enteric nerve fibers containing both nitric oxide synthase and galanin immunoreactivities in the striated muscle of the rat esophagus","volume":"295","author":"Kuramoto H","year":"1999","journal-title":"Cell Tissue Res"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1982.tb07101.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90265-9"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051153"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.274.5.R1425"},{"key":"e_1_2_6_36_1","first-page":"351","article-title":"The dual nature of the tachykinin NK1 receptor","volume":"18","author":"Maggi CA","year":"1997","journal-title":"TiPS"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050846"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00354780"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(98)00034-4"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(92)90125-M"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960408)367:3<342::AID-CNE2>3.0.CO;2-5"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(85)90505-0"},{"key":"e_1_2_6_43_1","series-title":"Handbook of experimental pharmacology","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/978-3-642-68437-1_9","author":"Roman C","year":"1982"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(91)90379-R"},{"key":"e_1_2_6_45_1","first-page":"7","article-title":"Substance P activates rat colonic motility via excitatory and inhibitory neural pathways and direct action on muscles","volume":"271","author":"Scheurer U","year":"1994","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.2000.00199.x"},{"key":"e_1_2_6_47_1","first-page":"482","article-title":"Agonist\u2010independent, muscle\u2010type\u2010specific signal transduction pathways in cat esophageal and lower esophageal sphincter circular smooth muscle","volume":"273","author":"Sohn UD","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903580406"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(01)00290-9"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00260977"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(84)90374-4"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(93)90049-G"},{"key":"e_1_2_6_53_1","first-page":"577","article-title":"Experimental colchicine encephalopathy. I. Introduction of neurofibrillary degeneration","volume":"17","author":"Wisniewski H","year":"1967","journal-title":"Lab Invest"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(94)90169-4"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(02)00293-X"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.20169","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.20169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T00:12:52Z","timestamp":1700266372000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.20169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8,20]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,10,4]]}},"alternative-id":["10.1002\/cne.20169"],"URL":"https:\/\/doi.org\/10.1002\/cne.20169","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8,20]]}}}