{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:45:21Z","timestamp":1790095521797,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T00:00:00Z","timestamp":1092009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2004,9,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently purified a peptide with structural features similar to vertebrate gonadotropin\u2010releasing hormone (GnRH) from the brain of <jats:italic>Octopus vulgaris<\/jats:italic>, cloned a cDNA encoding the precursor protein, and named it oct\u2010GnRH. In the current study, we investigated the expression and distribution of oct\u2010GnRH throughout the central nervous system (CNS) and peripheral organs of <jats:italic>Octopus<\/jats:italic> by in situ hybridization on the basis of the cDNA sequence and by immunohistochemistry using a specific antiserum against oct\u2010GnRH. Oct\u2010GnRH mRNA\u2010expressing cell bodies were located in 10 of 19 lobes in the supraesophageal and subesophageal parts of the CNS. Several oct\u2010GnRH\u2013like immunoreactive fibers were seen in all the neuropils of the CNS lobes. The sites of oct\u2010GnRH mRNA expression and the mature peptide distribution were consistent with each other as judged by in situ hybridization and immunohistochemistry. In addition, many immunoreactive fibers were distributed in peripheral organs such as the heart, the oviduct, and the oviducal gland. Modulatory effects of oct\u2010GnRH on the contractions of the heart and the oviduct were demonstrated. The results suggested that, in the context of reproduction, oct\u2010GnRH is a key peptide in the subpedunculate lobe and\/or posterior olfactory lobe\u2013optic gland\u2013gonadal axis, an octopus analogue of the hypothalamo\u2013hypophysial\u2013gonadal axis. It may also act as a modulatory factor in controlling higher brain functions such as feeding, memory, movement, maturation, and autonomic functions. J. Comp. Neurol. 477:310\u2013323, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.20260","type":"journal-article","created":{"date-parts":[[2004,8,10]],"date-time":"2004-08-10T07:21:26Z","timestamp":1092122486000},"page":"310-323","source":"Crossref","is-referenced-by-count":103,"title":["Expression and distribution of octopus gonadotropin\u2010releasing hormone in the central nervous system and peripheral organs of the octopus (<i>Octopus vulgaris<\/i>) by in situ hybridization and immunohistochemistry"],"prefix":"10.1002","volume":"477","author":[{"given":"Eiko","family":"Iwakoshi\u2010Ukena","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Ukena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Takuwa\u2010Kuroda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atshuhiro","family":"Kanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Tsutsui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Minakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-0216"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.2000.7524"},{"key":"e_1_2_6_4_1","series-title":"Neurobiology and behavior, part 2: the mollusca","first-page":"1","author":"Boyle PR","year":"1986"},{"key":"e_1_2_6_5_1","series-title":"The microscopic anatomy of invertebrates: mollusca II","first-page":"119","author":"Budelmann BU","year":"1997"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.1.278"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.141.2.505"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980817)398:1<1::AID-CNE1>3.0.CO;2-5"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.90000"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040549097"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.1999.0181"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(74)90747-1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1098\/rspb.1979.0071","article-title":"Antigen\u2010induced secretion in the optic gland of Octopus vulgaris","volume":"205","author":"Froesch D","year":"1979","journal-title":"Proc R Soc Lond B Biol Sci"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00361712"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1975.0005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/BF01921936","article-title":"On the turnover of exogenous ferritin in the cephalopod optic gland. 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