{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:27:35Z","timestamp":1791293255301,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T00:00:00Z","timestamp":1170374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mutations in the gene encoding methyl CpG binding protein 2 (MeCP2) are the primary cause of the neurodevelopmental disorder Rett syndrome (RTT). Mecp2\u2010deficient mice develop a neurological phenotype that recapitulates many of the symptoms of RTT, including postnatal onset of the neurological deficits. MeCP2 has two isoforms, MeCP2e1 and MeCP2e2, with distinct amino termini, which are generated by alternative splicing. We examined the distribution of the <jats:italic>Mecp2<\/jats:italic> splice variants in the postnatal mouse brain by in situ hybridization and found regional and age\u2010related differences in transcript abundance. In newborn mice, signals for total <jats:italic>Mecp2<\/jats:italic> and the <jats:italic>Mecp2e2<\/jats:italic> transcripts were widely distributed, with overlapping expression patterns throughout the brain. Expression of the <jats:italic>Mecp2e2<\/jats:italic> splice variant became largely restricted to nuclei within the dorsal thalamus (DT) and cortical layer V in juvenile animals, a pattern that was maintained into adulthood. In contrast, the total <jats:italic>Mecp2<\/jats:italic> riboprobe only weakly labeled the DT and cortical layer V in juvenile and adult animals, although it heavily labeled surrounding brain regions, suggesting that <jats:italic>Mecp2e1<\/jats:italic> is the predominant transcript outside the thalamus. Quantitative real\u2010time PCR was used to measure <jats:italic>Mecp2e1<\/jats:italic> and <jats:italic>Mecp2e2<\/jats:italic> abundance in the diencephalon of adult mice, demonstrating significantly more <jats:italic>Mecp2e2<\/jats:italic> in the DT than in the hypothalamus, which is in agreement with the <jats:italic>Mecp2e2<\/jats:italic> in situ hybridization. The differential distribution of the <jats:italic>Mecp2e1<\/jats:italic> and <jats:italic>Mecp2e2<\/jats:italic> transcripts indicates regional and developmental regulation of <jats:italic>Mecp2<\/jats:italic> splicing in the postnatal mouse brain. J. Comp. Neurol. 501:526\u2013542, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.21264","type":"journal-article","created":{"date-parts":[[2007,2,4]],"date-time":"2007-02-04T02:53:58Z","timestamp":1170557638000},"page":"526-542","source":"Crossref","is-referenced-by-count":94,"title":["Differential distribution of the <i>MeCP2<\/i> splice variants in the postnatal mouse brain"],"prefix":"10.1002","volume":"501","author":[{"given":"Joanna M.","family":"Dragich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Hwan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur P.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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