{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:07:18Z","timestamp":1771456038662,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T00:00:00Z","timestamp":1175126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Approximately 90% of fetal alcohol syndrome cases are accompanied by ocular abnormalities. The zebrafish (<jats:italic>Danio rerio<\/jats:italic>) is a well\u2010known developmental model that provides an opportunity for better understanding the histological and cytological effects of developmental exposure to ethanol on the vertebrate eye. The purpose of the present study was to determine the gross, microscopic, and ultrastructual effects of developmental exposure to ethanol in the zebrafish model. Eggs were obtained from WT outbred zebrafish and exposed to 0%, 0.1%, 0.2%, 0.4%, 0.5%, or 1.0% (v\/v) ethanol to assess viability and the effect of dose and duration of exposure on eye size. Light and electron microscopy were performed on ethanol\u2010treated and control larvae. Results showed that ethanol treatment decreased viability by about 20% at concentrations of 0.1\u20130.5% ethanol and by 50% at 1.0% ethanol. Ethanol\u2010related decreases in eye size were recorded at 6 days postfertilization (dpf) and were dose dependent. There were significant decreases in the volumes of the photoreceptor, inner nuclear, and ganglionic layers and in the lens of 9 dpf ethanol\u2010exposed compared with control larvae. Ultrastructural examination showed signs of developmental lags in the ethanol\u2010treated fish as well as abnormal retinal apoptosis in the 6 dpf ethanol\u2010treated larvae compared with their controls. These results demonstrate that the developing zebrafish eye is sensitive to perturbation with ethanol and displays some of the eye deficits present in fetal alcohol syndrome. J. Comp. Neurol. 502:497\u2013506, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.21320","type":"journal-article","created":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T16:50:55Z","timestamp":1175187055000},"page":"497-506","source":"Crossref","is-referenced-by-count":48,"title":["Ocular deficits associated with alcohol exposure during zebrafish development"],"prefix":"10.1002","volume":"502","author":[{"given":"Cynthia A.","family":"Dlugos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Rabin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/0892-0362(95)00005-C","article-title":"An update on the incidence of FAS: FAS is not an equal opportunity birth defect","volume":"17","author":"Abel 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