{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:58:04Z","timestamp":1790913484661,"version":"4.1.0"},"reference-count":87,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,10,9]],"date-time":"2007-10-09T00:00:00Z","timestamp":1191888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2007,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Olfactory ensheathing cells (OECs) are promising candidates for autologous cell transplantation therapies of nervous system injury and disease. Large animal models are relevant for transferring experimental data into clinical practice. In vivo studies have suggested that adult canine OECs may display similar regenerating capacities as their rodent counterpart. However, data on their molecular phenotype required for generating pure cell preparations are still scarce. In the present study, we comparatively analyzed expression of the carbohydrate HNK\u20101 epitope and the neurotrophin receptor p75<jats:sup>NTR<\/jats:sup> in adult canine Schwann cells and olfactory ensheathing cells in situ and in vitro. Myelinating and nonmyelinating Schwann cells in situ exclusively expressed HNK\u20101 and p75<jats:sup>NTR<\/jats:sup>, respectively, whereas OECs were negative for both markers. In vitro, OECs and Schwann cells shared cell surface expression of p75<jats:sup>NTR<\/jats:sup> but not of HNK\u20101, which could be detected transiently in intracellular vesicles. This suggests that Schwann cells and OECs in vitro phagozytose HNK\u20101<jats:sup>+<\/jats:sup> cellular debris. The cultivation\u2010induced downregulation of HNK\u20101 expression in Schwann cells and upregulation of p75<jats:sup>NTR<\/jats:sup> in OECs argues for the possibility that axonal signals control the expression of both markers in situ. Whereas HNK\u20101 expression in Schwann cells is most likely controlled by signals inducing myelination, e.g., neuregulin, the mechanisms that may suppress p75<jats:sup>NTR<\/jats:sup> expression in OECs in situ remain to be elucidated. Interestingly, HNK\u20101 expression in the adult dog was found in both sensory and motor nerve myelinating Schwann cells. This is reminiscent of humans and differs from rodents; it also underscores the importance of large animal models for translational research. J. Comp. Neurol. 505:572\u2013585, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.21519","type":"journal-article","created":{"date-parts":[[2007,10,9]],"date-time":"2007-10-09T20:18:48Z","timestamp":1191961128000},"page":"572-585","source":"Crossref","is-referenced-by-count":47,"title":["Differential expression of HNK\u20101 and p75<sup>NTR<\/sup> in adult canine Schwann cells and olfactory ensheathing cells in situ but not in 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