{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T16:39:36Z","timestamp":1717173576101},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,6]],"date-time":"2009-01-06T00:00:00Z","timestamp":1231200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2009,3,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molecular studies have revealed the presence of R\u2010type voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels at pre\u2010 and postsynaptic regions; however, no evidence for the participation of these channels in transmitter release has been presented for the spinal cord. Here we characterize the effects of SNX\u2010482, a selective R channel blocker, on the monosynaptic excitatory postsynaptic potentials (EPSPs) evoked in motoneurons by stimulation of dorsolateral funiculus (DLF) terminals in a slice preparation from the adult turtle spinal cord. SNX\u2010482 inhibited neurotransmission in a dose\u2010dependent manner, with an IC<jats:sub>50<\/jats:sub> of \u223c9 \u00b1 1 nM. The EPSP time course and membrane time constant of the motoneurons were not altered, suggesting a presynaptic mechanism. The toxin inhibited the residual component of the EPSPs recorded in the presence of N\u2010 and P\/Q\u2010type Ca<jats:sup>2+<\/jats:sup> channel blockers, strongly suggesting a role for the R channels in neurotransmission at the spinal cord DLF terminals. Consistently with this, RT\u2010PCR analysis of turtle spinal cord segments revealed the expression of the Ca<jats:sub>V<\/jats:sub>2.3 pore\u2010forming (\u03b1<jats:sub>1E<\/jats:sub>) subunit of R channels, whereas the use of anti\u2010\u03b1<jats:sub>1E<\/jats:sub>\u2010specific antibodies resulted in its localization in the DLF fibers as demonstrated by immunohistochemistry coupled with laser confocal microscopy. J. Comp. Neurol. 513:188\u2013196, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.21952","type":"journal-article","created":{"date-parts":[[2009,1,28]],"date-time":"2009-01-28T05:30:36Z","timestamp":1233120636000},"page":"188-196","source":"Crossref","is-referenced-by-count":4,"title":["Involvement of R\u2010type Ca<sup>2+<\/sup> channels in neurotransmitter release from spinal dorsolateral funiculus terminals synapsing 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