{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:47:28Z","timestamp":1791283648679,"version":"4.1.0"},"reference-count":86,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Dogs may naturally suffer an age\u2010related cognitive impairment that has aroused a great deal of interest, even beyond the field of the veterinary clinic. This canine senile dementia reproduces several key aspects of Alzheimer's disease (AD), including the presence of \u03b2\u2010amyloid (A\u03b2) deposits in the cerebral cortex, neurodegeneration, and learning and memory impairments. In the present study, we have used unbiased stereological procedures to estimate the number of the dorsal and median raphe nuclei (DRN and MRN, respectively) serotonergic neurons immunolabeled with an anti\u2010tryptophan hydroxylase (TrH) monoclonal antibody in young and aged dogs without A\u03b2 cortical deposits and in aged dogs with A\u03b2 cortical deposits. The estimated total number of TrH\u2010labeled neurons (mean \u00b1 SD) was 94,790 \u00b1 26,341 for the DRN and 40,404 \u00b1 8,692 for the MRN. The statistical analyses revealed that aged dogs with A\u03b2 cortical pathology had 33% fewer serotonergic neurons in the DRN and MRN than aged dogs without A\u03b2 cortical deposits (108,043 \u00b1 18,800 vs. 162,242 \u00b1 39,942, respectively;\n                    <jats:italic>P<\/jats:italic>\n                    = 0.01). In contrast, no significant variations were found between young and aged dogs without A\u03b2 cortical deposits. These results suggest that degeneration of the serotonergic neurons could be involved in the cognitive damage that accompanies A\u03b2 cortical pathology in the dog and reinforce the use of the canine model for exploring the potential mechanisms linking the cortical A\u03b2 pathology and serotonergic neurodegeneration that occurs during the course of AD. J. Comp. Neurol. 513:417\u2013429, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cne.21985","type":"journal-article","created":{"date-parts":[[2009,1,30]],"date-time":"2009-01-30T17:11:27Z","timestamp":1233335487000},"page":"417-429","source":"Crossref","is-referenced-by-count":40,"title":["\u03b2\u2010amyloid cortical deposits are accompanied by the loss of serotonergic neurons in the dog"],"prefix":"10.1002","volume":"513","author":[{"given":"Vanessa","family":"Bernedo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Insua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda\u2010Luisa","family":"Su\u00e1rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germ\u00e1n","family":"Santamarina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Sarasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Pesini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-5846(00)00101-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(99)00132-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0197-4580(92)90073-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2004.12.008"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903010202"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890070407"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90043-N"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1996.tb01045.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-5846(00)00108-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90050-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90342-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902870308"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2000.00254.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(00)00113-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb16897.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/nlme.1996.0039"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0197-4580(95)02060-8"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-198407000-00001"},{"issue":"232","key":"e_1_2_6_20_1","first-page":"1","article-title":"Evidence of the existence of monoamine containing neurons in the central nervous system. 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